An automated droplet mixing chip with a single plane and a single electrode control method thereof

Abstract

The invention belongs to the technical field of automated chemical and biological instruments, and in particular relates to an automated droplet mixing chip with a single plane and a single electrode control method thereof. The automated droplet mixing chip with the single plane of the present invention includes two major parts of a droplet mixing zone and a signal input zone which are connected by circuits. A droplet control voltage is sequentially applied to each lead electrode of the single input zone, while voltages of remaining lead electrodes are maintained suspended or grounded, so that a plurality of reagent droplets are converged and mixed to produce biological or chemical reactions. The chip is cleverly designed with a very simple structure, and low cost; reagents can be dropped in very conveniently, and the use amount of reagent droplets is little, thus saving biochemical reagents and reducing testing costs. The automated droplet mixing chip with the single plane of the present invention can be widely used in portable automated chemical and biological detection instruments.

Description

technical field [0001] The invention belongs to the technical field of automated chemical and biological instruments, and in particular relates to a single-plane droplet automatic mixing chip and a single-electrode control method thereof. Background technique [0002] At present, the traditional two-dimensional digital microfluidic technology needs to place the droplet between the upper and lower control plates and apply voltage to make the droplet move between the two plates. This kind of control chip requires two upper and lower layers. The liquid droplet is sandwiched in the middle, so that the system complexity is high, the liquid droplet motion resistance is relatively large, and the liquid droplet does not have the effect of concentrating light. We previously applied for a transparent single-plane unipolar digital microfluidic chip patent (publication number CN102350380A), which discloses a method for realizing a single-plane two-dimensional double-layer chip. The chip...

AI Technical Summary

Problems solved by technology

The traditional droplet mixing method (refer to the patent "Automatic Mixing Liquid Delivery Device" Patent No.: 200820214694) requires the use of closed water pipes, mixing chambers, and power devices such as compressed air pipes or liquid pumps. Due to the use of a large number of Mechanical assembly, bulky and unsuitable for portable instruments

However, the traditional droplet mixing unit based on electrowetting digital microfluidic chip (publication number CN102836653A) based on the principle of electrowetting requires two upper and lower layers of chips, and the reaction droplets are sandwiched in the middle. The system structure is complex, and the droplet It is difficult to add reaction reagents, and it is not suitable for portable instruments

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