Method for quickly detecting duck-derived components in food

Abstract

The invention discloses a method for quickly detecting duck-derived components in food, which is a TaqMan probe fluorescent quantitative PCR (polymerase chain reaction) method. The method comprises the following steps: by using a TaqMan probe containing a DNA (deoxyribonucleic acid) template to be detected, a recombinant plasmid containing positive amplification internal standard DNA and the positive amplification internal standard DNA as well as an IL-2 gene amplification primer and a TaqMan probe for ducks, performing fluorescent quantitative PCR reaction; and performing analysis processing on the results by using ABI7500 Software SDS1.4, wherein the amplification of which the Ct value is less than 36 is taken as a positive detection result. The method firstly proposes that duck-derived components in food can be detected through the fluorescent quantitative PCR method by taking a gene in a karyon as a target gene and adding a competitive amplification internal standard. The method has favorable specificity, can avoid the false negative result by monitoring the PCR reaction in real time, explores a new way to identify animal-derived components in food, and has the advantages of accuracy, stability, convenient operation process and the like.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and relates to a method for rapidly detecting duck-derived components in food, in particular to a rapid detection method for duck-derived components in food with a TaqMan probe added with an amplified internal standard by real-time fluorescent PCR. Background technique [0002] In recent years, the quality and safety of meat products has become a hot topic of concern to the whole society. Driven by economic interests, some lawbreakers adulterated and sold relatively cheap duck and pork as beef and mutton in order to make huge profits. These behaviors not only damaged the quality of cattle and sheep products, but also caused great harm to Muslims. , it is very likely that the introduction of allergens will endanger the health of consumers, and it will also damage the international reputation of cattle and sheep products. The "horse meat crisis" that occurred in many European countrie...

AI Technical Summary

Problems solved by technology

In terms of target gene selection, animal cell nuclear genomic DNA sequences are highly species-specific and can avoid non-specific amplification; in addition, the current research on the detection of animal-derived real-time PCR in food lacks a positive amplification internal standard (IAC) Monitoring the reaction system cannot avoid false negative results, resulting in inaccurate test results

At present, there are no reports on animal-derived fluorescent quantitative PCR detection methods in food that use the DNA sequence of the cell nucleus gene as the target gene and add an internal standard for amplification.

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