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Method for rapid detection of giant panda estradiol concentration value

A detection method and technology for giant pandas, which are applied to measurement devices, material analysis by optical means, instruments, etc., can solve the problems of shortening the detection time of female giant panda urine samples, and achieve the effect of shortening the detection time.

Inactive Publication Date: 2013-07-24
CHENGDU RES BASE OF GIANT PANDA BREEDING
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is a rapid detection method for the concentration of estradiol in giant pandas that shortens the detection time of female giant panda urine samples and ensures that giant pandas have sufficient natural mating time or artificial insemination time

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  • Method for rapid detection of giant panda estradiol concentration value
  • Method for rapid detection of giant panda estradiol concentration value
  • Method for rapid detection of giant panda estradiol concentration value

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Embodiment Construction

[0030] In the present invention, during the spring breeding period, after cleaning and drying the floor of the animal house, put the female giant panda that needs to collect urine samples into the animal house; then the staff waits, and once the giant panda urinates, immediately put the giant panda into another animal house , use a clean syringe to collect a fresh urine sample from the ground, put it into a cryovial, mark it, and immediately put it in the freezer of the refrigerator for freezing or storage in the refrigerator.

[0031] Divide the same urine sample into multiple small portions, usually at least three small portions.

[0032] When detecting the value of estradiol, first coat the 96-well ELISA plate overnight with estrogen antibody R522-2 for 6-48 hours; Do a series of equal dilutions and place them in sequence figure 1 In the detection wells S1 to S10 on the 96-well ELISA plate shown; make a small urine sample according to the ratio of 1:20, 1:40, 1:80, 1:160, ...

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Abstract

The invention discloses a method for rapid detection of a giant panda estradiol concentration value. Under the premise of no urine sample detection result difference, the tradition way of incubation at room temperature for 2h is changed to incubation for 0.5h at 37DEG C, so that the total detection process of the giant panda estradiol concentration value is shortened from the original 5h to 2h, and the detection time is shortened by 60%, thus winning precious time for timely natural mating and artificial insemination.

Description

technical field [0001] The invention relates to a detection method for estrogen changes in female animals in estrus, in particular to a rapid detection method for the concentration value of estradiol in giant pandas. Background technique [0002] As a rare and endangered wild animal unique to my country, giant pandas have attracted a large number of researchers to study them due to their unique physiological characteristics. Female giant pandas are cyclical single-estrous animals, mostly in spring and autumn, and only accept natural mating for 1 to 3 days in a single estrous period. In captivity, only about 50% of giant pandas mate naturally, and about half of them need to be bred through artificial insemination. Judging whether giant pandas are ovulating in the shortest time and performing artificial insemination is the key to successful breeding of giant pandas in captivity. [0003] In the early breeding of giant pandas in captivity, the most important basis for judging...

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Application Information

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IPC IPC(8): G01N21/17
Inventor 罗娌侯蓉张志和黄河王成东兰景超黄祥明
Owner CHENGDU RES BASE OF GIANT PANDA BREEDING
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