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Blue-green alga-rice complex operation nitrogen-fixing and carbon-fixing method

A nitrogen fixation and cyanobacteria technology, applied in the biological field, to achieve great theoretical value and application potential, simple process, and the effect of improving yield and quality

Inactive Publication Date: 2013-07-24
SHANGHAI JIAO TONG UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there are very few related studies on Anabaena spp., Nitrogen-fixing Anabaena, and Anabaena blooms at home and abroad. The Chinese Academy of Sciences once mentioned in "Absorption Spectrum and Fluorescence Spectrum Characteristics of Dormant and Germinated Spores of Anabaena spp. The induction method of spore content, in the production of nitrogen-fixing cyanobacteria and the preparation of dry algae powder, only see Shen Jianying et al. Rapid activation method and mass production method of dried algae powder", "Preparation method of dried algae powder of Manjiang red Anabaena", and Shen Yinwu et al. mentioned in "Results and Prospects of Nitrogen-fixing Cyanobacteria Cultivation and Application", while in other aspects Exploration is rarely reported

Method used

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  • Blue-green alga-rice complex operation nitrogen-fixing and carbon-fixing method

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Experimental program
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Effect test

Embodiment 1

[0036] Main materials and equipment: three kinds of nitrogen-fixing cyanobacteria (Manjiang red Anabaena, nitrogen-fixing Anabaena, and bloom Anabaena), sieves, open containers, stirring rods, ovens, balances, and small ponds in greenhouses.

[0037] The specific steps are:

[0038] 1) Using Manjiang red Anabaena, Nitrogen-fixing Anabaena and Bloom Anabaena as initial materials, first expand the algae species in a sterile room, and the process conditions are: light intensity 3000±200Lx, temperature 30±2℃; then The algae cultured in the sterile room were inoculated into large-scale production in small greenhouses (length 2m×width 0.5m×height 0.8m), the temperature was 30±2°C, natural light was added, and 4g of algae with a water content of 50% was added to each pool. Cultivate continuously for 4 days; then control the water with the help of sieves, nitrogen-fixing Anabaena and Anabaena spp. pass through a 300-mesh sieve, and bloom Anabaena pass through a 50-mesh sieve to harves...

Embodiment 2

[0043] Main materials and equipment: three kinds of nitrogen-fixing cyanobacteria (Manjiang red Anabaena, nitrogen-fixing Anabaena, and bloom Anabaena), sieves, open containers, stirring rods, ovens, balances, and small ponds in greenhouses.

[0044] The concrete steps of the present embodiment are except following technological conditions, and all the other content and step process are identical with above-mentioned embodiment 1:

[0045] The size of the small pool in the greenhouse is 1.5m long x 1m wide x 1.2m high. Add 5g of algae species with a water content of 50% to each pool, and cultivate continuously for 5 days. With the help of sieves, the water is controlled. Nitrogen-fixing anabaena and red anabaena pass through a 250 mesh sieve. Anabaena blooms pass through an 80-mesh sieve to harvest wet algae with a water content of 60%; the wet algae of the three nitrogen-fixing cyanobacteria follow the mass ratio of red Anabaena: Nitrogen-fixing Anabaena: bloom Anabaena: 2.5:5...

Embodiment 3

[0047] Main materials and equipment: three kinds of nitrogen-fixing cyanobacteria (Manjiang red Anabaena, nitrogen-fixing Anabaena, and bloom Anabaena), sieves, open containers, stirring rods, ovens, balances, and small ponds in greenhouses.

[0048] The concrete steps of the present embodiment are except following technological conditions, and all the other content and step process are identical with above-mentioned embodiment 1:

[0049] The size of the small pools in the greenhouse is 1m long x 0.8m wide x 1m high. Add 2g of algae species with a water content of 50% to each pool, and cultivate continuously for 3 days. With the help of sieves, the water is controlled. Anabaena chinensis passes through a 100-mesh sieve to harvest wet algae with a water content of 40%; the wet algae of three nitrogen-fixing cyanobacteria are mixed according to the mass ratio of Manjiang red Anabaena: nitrogen-fixing Anabaena: water bloom Anabaena 3:4:3 The mixed nitrogen-fixing cyanobacteria i...

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Abstract

The invention relates to a blue-green alga-rice complex operation nitrogen-fixing and carbon-fixing method. Anabaena azollae, anabaena sphaerica and anabaena flosaguas are taken as initial materials, and after expanded culture of strains in a sterile chamber and scale production in a small pool of a greenhouse, the strains are processed with the help of a screen to enable the water content to be 40%-60%; then mixing, stirring and crushing are performed at the day according to the weight ratio of 2: 6: (2-3): 4: 3, and a mixture is uniformly sprayed into a watered rice field with the density of 2-5g / m<2>; or wet algae are placed in an oven at the temperature of 35-40 DEG C for 8h or placed under a fluorescent lamp of 30-50W in the sterile chamber for drying for 3-5 days to enable the water content to be 8%-12%, crushing is performed by using a crushing machine, dry alga powder is uniformly blended according to the weight ratio of 1: 8: (1-2): 6: 2 and mixed in 5-10 times of the weight of rice husk, and the dry alga powder is uniformly sprayed into the watered rice field with the density of 0.5g / m<2>. According to the method disclosed by the invention, nitrogen-fixing blue-green algae are cultivated and stocked in the rice field as a fertilizer source, CO2 is absorbed, organic matters are generated, soil is improved, the yield and quality of paddy rice are improved, and the method has the advantages of simple process, easiness in popularization, safety, high efficiency, no pollution and the like.

Description

technical field [0001] The invention relates to a method for preparing and applying agricultural biological bacterial fertilizer, in particular to a method for fixing nitrogen and carbon in the compound management of cyanobacteria and rice, and belongs to the field of biotechnology. Background technique [0002] Anabaena azollae (No.: FACHB-190), Nitrogen-fixing Anabaena (Anabaena azollae, No. FACHB-686), and Anabaena flos-aquae (No. FACHB-245) were approved by the Chinese Academy of Sciences. Provided by the freshwater algae species bank of the Institute of Hydrobiology, and has been published in "Jianying Shen, Antonio DiTommaso, Mingquan Shen, Wei Lu, and Zhengming Li; Molecular basis for differential metabolic responses tomonosulfuron in three nitrogen-fixing cyanobacteria, Weed Science, 2009, 57 : 178-188", "Jianying Shen, Jing Jiang, Peizhong Zheng; Effects of Light and Monosulfuron on growth and Photosynthetic Pigments of Anabaena flos-aquae Breb, Water Resource and P...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01G1/00A01G7/00C12R1/01
Inventor 沈健英万旗东
Owner SHANGHAI JIAO TONG UNIV
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