Callicarpa kwangtungensis chun. low-polarity ingredient extract, pharmaceutical composition containing Callicarpa kwangtungensis chun. low-polarity ingredient extract, preparation method and application

A low-polarity, extractive technology, applied in drug combinations, medical preparations containing active ingredients, pharmaceutical formulations, etc., can solve the problem of in vitro antibacterial activity without the low-polarity ingredients of Guangdong Zizhu

Active Publication Date: 2014-12-17
JIANGSU KANION PHARMA CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1. Kim Y S, Shin D H. Volatile Constituents from the Leaves of Callicarpajaponica Thunb. and Their Antibacterial Activities. J Agric FoodChem, 2004, 52(4): 781-787. The antibacterial activity of the volatile components of Guangdong Zizhu against six food-borne microorganisms, but there is no description of the preparation of the low-polarity components of Guangdong Zizhu and their in vitro antibacterial activity
[0006] 2. Li Deying, Yuan Huide. Comparison of composition, toxicity and antibacterial effect of Huazizhu and Duhonghua. Modern Applied Pharmacy. 1992, 9(1): 13-15. Determination of antibacterial activity of chemical constituents, but there is no description of the preparation of the low-polarity constituents of Guangdong Zizhu and their in vitro antibacterial activity
[0007] 3. Hong Yanlong. Preparation process, quality standard and chemical composition of Guangdong Zizhu effervescent tablets. Beijing: Beijing University of Traditional Chinese Medicine, 2004. This article verified the anti-inflammatory activity of Guangdong Zizhu total flavonoids by in vivo experiments , but there is no description of the preparation of the low-polarity components of Guangdong Zizhu and its in vitro antibacterial activity

Method used

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  • Callicarpa kwangtungensis chun. low-polarity ingredient extract, pharmaceutical composition containing Callicarpa kwangtungensis chun. low-polarity ingredient extract, preparation method and application
  • Callicarpa kwangtungensis chun. low-polarity ingredient extract, pharmaceutical composition containing Callicarpa kwangtungensis chun. low-polarity ingredient extract, preparation method and application
  • Callicarpa kwangtungensis chun. low-polarity ingredient extract, pharmaceutical composition containing Callicarpa kwangtungensis chun. low-polarity ingredient extract, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Supercritical CO at the low polarity part of Guangdong Zizhu 2 preparation

[0038] Guangdong Zizhu medicinal material is crushed, passed through a 24-mesh sieve, weighed 200g, placed in a supercritical 1L extraction kettle (extraction kettle II), and extracted according to the following conditions: extraction temperature is 45°C, pressure 25Mpa; separation kettle temperature is 50°C, pressure 8Mpa ;CO 2 Flow rate 50L / h, total flow 100L; extraction time 2h. The extract from the separation kettle was collected, and the extract was a brown-yellow oily substance. The sample in the separation kettle was extracted with anhydrous ether, and dehydrated with an appropriate amount of anhydrous sodium sulfate to obtain a sample with a yield of 1.042%.

Embodiment 2

[0039] Example 2: Supercritical CO at the low polarity part of Guangdong Zizhu 2 preparation

[0040] Guangdong Zizhu medicinal material is crushed, passed through a 24-mesh sieve, weighed 200g, placed in a supercritical 1L extraction kettle (extraction kettle II), and extracted according to the following conditions: extraction temperature is 35°C, pressure 25Mpa; separation kettle temperature is 30°C, pressure 5.7 Mpa; CO 2 The flow rate is 50L / h, the total flow rate is 100L; the extraction time is 1.5h. Collect the extract from the separation tank, the extract is a light yellow liquid sample, extract the sample from the separation tank with n-hexane, dehydrate with an appropriate amount of anhydrous sodium sulfate to obtain the sample, and the yield is 0.658%.

Embodiment 3

[0041] Embodiment 3: GC-MS analysis of the chemical composition of the Guangdong purple pearl low polarity component extract obtained in embodiment 1

[0042] 1) GC-MS chromatographic conditions

[0043] Chromatographic column HP-5 capillary column (30m×0.25mm×0.25μm); high-purity nitrogen is used as the carrier gas, the temperature of the injection port is 300°C, the injection method is split injection, the split ratio is 30:1, the injection volume is 1μl, and the Gas flow rate 1.0ml / min; temperature program: initial temperature 200°C, keep for 3min, then rise to 250°C at 4°C / min, keep for 5min, then rise to 300°C at 20°C / min, keep for 3min. The mass spectrometry interface temperature is 310°C, the EI source temperature is 200°C, the electron bombardment energy is 60eV, the scanning range is m / z 25-550amu, all ion scanning, the acquisition delay is 5.0min, and the relative content is calculated by the normalization method.

[0044] The GC-MS total ion chromatogram of embodim...

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Abstract

The invention provides a Callicarpa kwangtungensis chun. low-polarity ingredient extract, which is characterized by comprising 15-28% of aliphatic acid compound, 30-45% of hydrocarbon compound and 2-8% of terpene component. The invention also provides a preparation method for a Callicarpa kwangtungensis chun. essential oil extract, a pharmaceutical composition containing the Callicarpa kwangtungensis chun. essential oil extract, and an application.

Description

technical field [0001] The invention belongs to the pharmaceutical field of traditional Chinese medicine or natural medicine, and in particular relates to a low-polar component extract of Guangdong purple pearl, a preparation method thereof, a pharmaceutical composition containing the volatile oil extract of Guangdong purple pearl and its use for inhibiting cervicitis pathogenic bacteria the use of. Background technique [0002] The survey of Chinese women by the World Health Organization shows that the population of women of childbearing age in my country is about 150-200 million, of which about 41% of women suffer from gynecological inflammatory diseases to varying degrees, and the incidence rate of married women is as high as 70%. According to statistics, cervicitis accounts for about 40-50% of the total number of obstetrics and gynecology outpatients, ranking first among gynecological diseases, and chronic cervicitis accounts for 10%-25% of outpatients. Under normal cir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B9/02A61K36/85A61P15/00A61P31/04A61P31/10A61K127/00A61K135/00
CPCY02A50/30
Inventor 杨义芳贾安
Owner JIANGSU KANION PHARMA CO LTD
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