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Callicarpa kwangtungensis chun. low-polarity ingredient extract, pharmaceutical composition containing Callicarpa kwangtungensis chun. low-polarity ingredient extract, preparation method and application

A low-polarity, extractive technology, applied in the field of inhibiting cervicitis pathogenic bacteria, Guangdong Zizhu low-polar component extract, can solve the problem of no in vitro antibacterial activity of Guangdong Zizhu low-polar component

Active Publication Date: 2013-07-24
JIANGSU KANION PHARMA CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1. Kim Y S, Shin D H. Volatile Constituents from the Leaves of Callicarpajaponica Thunb. and Their Antibacterial Activities. J Agric FoodChem, 2004, 52(4): 781-787. The antibacterial activity of the volatile components of Guangdong Zizhu against six food-borne microorganisms, but there is no description of the preparation of the low-polarity components of Guangdong Zizhu and their in vitro antibacterial activity
[0006] 2. Li Deying, Yuan Huide. Comparison of composition, toxicity and antibacterial effect of Huazizhu and Duhonghua. Modern Applied Pharmacy. 1992, 9(1): 13-15. Determination of antibacterial activity of chemical constituents, but there is no description of the preparation of the low-polarity constituents of Guangdong Zizhu and their in vitro antibacterial activity
[0007] 3. Hong Yanlong. Preparation process, quality standard and chemical composition of Guangdong Zizhu effervescent tablets. Beijing: Beijing University of Traditional Chinese Medicine, 2004. This article verified the anti-inflammatory activity of Guangdong Zizhu total flavonoids by in vivo experiments , but there is no description of the preparation of the low-polarity components of Guangdong Zizhu and its in vitro antibacterial activity

Method used

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  • Callicarpa kwangtungensis chun. low-polarity ingredient extract, pharmaceutical composition containing Callicarpa kwangtungensis chun. low-polarity ingredient extract, preparation method and application
  • Callicarpa kwangtungensis chun. low-polarity ingredient extract, pharmaceutical composition containing Callicarpa kwangtungensis chun. low-polarity ingredient extract, preparation method and application
  • Callicarpa kwangtungensis chun. low-polarity ingredient extract, pharmaceutical composition containing Callicarpa kwangtungensis chun. low-polarity ingredient extract, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Supercritical CO at the low polarity site of Guangdong Zizhu 2 preparation

[0038] Guangdong Zizhu medicinal materials were pulverized, passed through a 24-mesh sieve, weighed 200g, placed in a supercritical 1L extraction kettle (extraction kettle II), and extracted according to the following conditions: the extraction temperature was 45°C, the pressure was 25Mpa; the temperature of the separation kettle was 50°C, and the pressure was 8Mpa ;CO 2 The flow rate is 50L / h, the total flow is 100L; the extraction time is 2h. The extract of the separation kettle was collected, and the extract was a brownish yellow oil. The sample of the separation kettle was extracted with anhydrous ether, and an appropriate amount of anhydrous sodium sulfate was dehydrated to obtain the sample, and the yield was 1.042%.

Embodiment 2

[0039] Example 2: Supercritical CO at the low polarity site of Guangdong Zizhu 2 preparation

[0040] Guangdong Zizhu medicinal materials were pulverized, passed through a 24-mesh sieve, weighed 200g, placed in a supercritical 1L extraction kettle (extraction kettle II), and extracted according to the following conditions: the extraction temperature was 35°C, and the pressure was 25Mpa; the separation kettle temperature was 30°C, and the pressure was 5.7 Mpa; CO 2 The flow rate is 50L / h, the total flow is 100L; the extraction time is 1.5h. The extract from the separation kettle was collected, and the extract was a light yellow liquid sample. The sample from the separation kettle was extracted with n-hexane, and an appropriate amount of anhydrous sodium sulfate was dehydrated to obtain a sample with a yield of 0.658%.

Embodiment 3

[0041] Example 3: GC-MS analysis of the chemical constituents of the Guangdong Zizhu low-polar constituent extract obtained in Example 1

[0042] 1) GC-MS chromatographic conditions

[0043] Chromatographic column HP-5 capillary column (30m×0.25mm×0.25μm); high-purity nitrogen gas as carrier gas, injection port temperature 300℃, injection mode split injection, split ratio 30:1, injection volume 1μl, load Air flow rate 1.0ml / min; temperature program: the initial temperature was 200°C, maintained for 3min, then increased to 250°C at 4°C / min, maintained for 5min, then increased to 300°C at 20°C / min for 3min. The mass spectrometer interface temperature was 310 °C, the EI source temperature was 200 °C, the electron bombardment energy was 60 eV, the scanning range was m / z 25-550 amu, the whole ion scan, the acquisition delay was 5.0 min, and the relative content was calculated by the normalization method.

[0044] The GC-MS total ion chromatogram of the low-polar component extract ...

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Abstract

The invention provides a Callicarpa kwangtungensis chun. low-polarity ingredient extract, which is characterized by comprising 15-28% of aliphatic acid compound, 30-45% of hydrocarbon compound and 2-8% of terpene component. The invention also provides a preparation method for a Callicarpa kwangtungensis chun. essential oil extract, a pharmaceutical composition containing the Callicarpa kwangtungensis chun. essential oil extract, and an application.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine or natural medicine pharmacy, and in particular relates to a low-polarity component extract of Guangdong Zizhu, a preparation method thereof, a pharmaceutical composition containing the Guangdong Zizhu volatile oil extract and its use for inhibiting cervicitis pathogenic bacteria the use of. Background technique [0002] The World Health Organization's survey of Chinese women shows that the population of women of childbearing age in my country is about 150-200 million, of which about 41% of women suffer from different degrees of gynecological inflammatory diseases, and the incidence rate of married women is as high as 70%. According to statistics, cervicitis accounts for about 40-50% of the total outpatient department of obstetrics and gynecology, ranking first in gynecological diseases, among which chronic cervicitis accounts for 10%-25% of outpatients. Under normal circumstances, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/85A61P15/00A61P31/04A61P31/10A61K127/00A61K135/00
CPCY02A50/30
Inventor 杨义芳贾安
Owner JIANGSU KANION PHARMA CO LTD
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