Quick panda progesterone concentration determination method

A detection method and technology for giant pandas, which can be applied in biological testing, material inspection products, etc., can solve the problem of shortening the detection time of female giant panda urine samples, and achieve the effect of shortening the detection time.

Inactive Publication Date: 2013-07-17
CHENGDU RES BASE OF GIANT PANDA BREEDING
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is a rapid detection method for the concentration of progesterone in giant pandas that shortens the detection time of female giant panda urine samples and ensures that giant pandas have sufficient natural mating time or artificial insemination time

Method used

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  • Quick panda progesterone concentration determination method
  • Quick panda progesterone concentration determination method
  • Quick panda progesterone concentration determination method

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Embodiment Construction

[0030] In the present invention, during the spring breeding period, after cleaning and drying the floor of the animal house, the female panda that needs to collect urine samples is put into the animal house; then the staff waits, once the panda urinates, immediately put the panda into other animal houses , Use a clean syringe to collect a fresh urine sample on the ground, put it into the freezing tube, make a mark, and immediately put it in the freezer of the refrigerator or cold storage in the refrigerator.

[0031] Divide the same urine sample into multiple small portions, usually at least three small portions.

[0032] When detecting the progesterone value, first coat the 96-well ELISA plate overnight with progesterone antibody CL425 for 6 to 48 hours; then make a series of 10 grades of progesterone standards in the concentration range of 0, 0.78 to 200 pg / well Dilute equally and place them in figure 1 In the detection wells S1 to S10 of the 96-well microtiter plate as shown; ma...

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Abstract

The invention discloses a quick panda progesterone concentration determination method. On the premise of no difference between detection results of urine samples, three hours for whole determination process of panda progesterone concentration is shortened to 1.5 hours by changing 2-hour incubation at conventional room temperature into 0.5-hour incubation at 37 DEG C, determination time is shortened by 50%, and precious time is earned for natural mating and artificial insemination in good time.

Description

Technical field [0001] The invention relates to a method for detecting changes in progesterone in female animals during estrus, and in particular to a method for quickly detecting the concentration of giant panda progesterone. Background technique [0002] As a rare and endangered wild animal unique to my country, the panda's unique physiological characteristics have attracted a large number of scientific researchers to study it. The female giant panda is a periodic single-estrus animal, most of which are estrus in spring and litter in autumn. They only accept natural mating for 1 to 3 days during an estrus period. Under captive conditions, only about 50% of giant pandas mate naturally, and about half of giant pandas need to be reproduced through artificial insemination. Judging whether a giant panda ovulates in the shortest time and performing artificial insemination is the key to the successful reproduction of giant pandas under captive conditions. [0003] In the early breedin...

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Application Information

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IPC IPC(8): G01N33/74
Inventor 罗娌侯蓉张志和黄河王成东黄祥明兰景超
Owner CHENGDU RES BASE OF GIANT PANDA BREEDING
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