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Split-range embryo culture solution and preparation method thereof

A technology of embryo culture solution and culture solution, which is applied in the field of cell culture, can solve the problems of harmful decomposition products, etc., and achieve the effects of improving growth and development in vitro, increasing success rate, and improving quality

Active Publication Date: 2015-02-04
南京优而生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The whole culture medium series process retains these important active ingredients, but also retains harmful decomposition products in the culture medium

Method used

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  • Split-range embryo culture solution and preparation method thereof
  • Split-range embryo culture solution and preparation method thereof
  • Split-range embryo culture solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A split-stage embryo culture medium consisting of cleavage fluid and blastocyst fluid. The cleavage fluid is used for fertilized eggs growing to the 8-cell stage, and the blastocyst fluid is used for fertilized eggs growing from the 8-cell stage to fertilization. During the pre-implantation period. Both the cleavage fluid and the blastocyst fluid are composed of basic culture fluid and embryo nutrition factors, and the components of the inorganic salt buffer in the basic culture fluid are the same.

[0028] The formula of the inorganic salt buffer solution is shown in Table 1, and its pH is between 7.3 and 7.5:

[0029] Table 1 The formula of cleavage fluid and blastocyst fluid

[0030]

[0031] Among them, amino acids include essential amino acids and non-essential amino acids, including essential amino acids and non-essential amino acids required by all embryos, and their content corresponds to the needs of embryos. Amino acids were purchased from Life Technologi...

Embodiment 2

[0037] The present embodiment is basically the same as Example 1, and the difference is that the formula of the basic culture solution is as shown in Table 2:

[0038] The formula of table 2 embodiment 2 basic culture fluid

[0039]

Embodiment 3

[0041] This embodiment is basically the same as Embodiment 1, and its difference is that the formula of embryonic culture fluid is as shown in table 3:

[0042] The formula of table 3 embodiment 3 basic culture fluid

[0043]

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Abstract

The invention discloses a split-range embryo culture solution and a preparation method thereof, belonging to the technical field of cytology and biology. The split-range culture solution added with three embryotrophic factors comprises a cleavage solution and a blastula solution, wherein the cleavage solution and the blastula are respectively used in a fertilized egg before and after 8-cell cycle. The embryotrophic factors added into the embryo culture solution disclosed by the invention have synergistic effect, so that the defect that the split-range culture solution loses the embryotrophic factors secreted by an embryo is avoided, the fertilized egg has higher embryo stem cell pluripotency during the growth in the culture solution disclosed by the invention, the development situation of the fertilized egg is equivalent to the in-vivo embryo growth, the in-vitro growth and development of the fertilized egg is improved, and the quality of the embryo is improved.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a split-stage embryo culture solution for improving the growth and development effect of fertilized eggs in vitro and a preparation method thereof. Background technique [0002] Since the world's first "test-tube" baby was born in the UK on July 25, 1978, as of July 1, 2012, 5 million "test-tube" babies have been born. IVF (in vitro fertilization) has brought good news to more than 50 million infertile couples worldwide. However, more than three decades of clinical practice has not significantly improved the success rate of IVF. In 2011, according to the American Pregnancy Association, the IVF success rate (pregnancy rate) for 35-year-old patients was around 25-30%, and it was around 30% in 1997. And the birth rate is even lower. One of the important reasons is the quality of IVF embryo culture medium. This is one of the most important determinants of IVF embryo quality. [0003]...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/073
Inventor 金星亮白丽君
Owner 南京优而生物科技发展有限公司
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