Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Anticancer application of sterols derivative

A use, cancer technology, applied in the field of medicine and chemical industry, can solve the problem of unknown anti-cancer ingredients and so on

Active Publication Date: 2013-06-26
BEIJING WBL PEKING UNIV BIOTECH
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in addition to lovastatin, there may be some unknown anti-cancer ingredients in Xuezhikang

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Anticancer application of sterols derivative
  • Anticancer application of sterols derivative
  • Anticancer application of sterols derivative

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0120] Embodiment 1: the preparation of formula II compound (1)

[0121] Steps:

[0122] 1) Take Xuezhikang Capsules (manufactured by Peking University Weixin) about 1 kg dry powder content, use 2-6 times the volume of dichloromethane as a solvent to ultrasonically extract 3 times, each time for 20-40 minutes, combine the extracts, concentrate under reduced pressure and The solvent was recovered to obtain 91 g of dichloromethane fine extract.

[0123] 2) 50 g of dichloromethane fine extract was taken, separated by silica gel column chromatography, and carried out gradient elution with petroleum ether and ethyl acetate. The volume ratio of petroleum ether-ethyl acetate is 75:25, 50:50, 25:75, 0:100 in sequence.

[0124] 3) Take 5.0 g of petroleum ether-ethyl acetate at a ratio of 25:75, separate it by C18 reverse-phase column chromatography, and obtain 4 fractions (methanol-water 10 :90, 50:50, 75:25, 100:0), wherein 1.3 g of methanol-water (75:25) eluting fraction was pur...

Embodiment 2

[0125] Embodiment 2: the preparation of formula II compound (2)

[0126] Steps:

[0127] 1) Take 5 kg of red yeast rice, use 2-6 times the volume of dichloromethane as a solvent for ultrasonic extraction 3 times, each time for 20-40 minutes, combine the extracts, concentrate under reduced pressure and recover the solvent to obtain 78 g of dichloromethane fine extract .

[0128] 2) Take 30 g of dichloromethane fine extract, and separate it by silica gel column chromatography, and carry out gradient elution with petroleum ether and ethyl acetate. The volume ratio of petroleum ether-ethyl acetate is 75:25, 50:50, 25:75, 0:100 in sequence.

[0129] 3) Take 3.0 g of petroleum ether-ethyl acetate at 25:75, separate by C18 reverse-phase column chromatography, and obtain 4 parts (methanol-water 10 :90, 50:50, 75:25, 100:0), wherein 0.8 g of methanol-water (75:25) eluting fraction was purified by semi-preparative high performance liquid chromatography and purified with acetonitril...

Embodiment 3

[0130] Embodiment 3: the structural identification of compound

[0131] The samples used were the compounds prepared in Examples 1 and 2.

[0132] 1. Physicochemical data of the compound

[0133] White powder, optical rotation: [α] 25 D -50.00 (c 0.118, CH 2 Cl 2 :MeOH=1:1);

[0134]There are three maximum absorption peaks in the UV spectrum, which are λ max (CH 2 Cl 2 : MeOH) = 271.6 nm, 282.2 nm, 293.8 nm.

[0135] FT-IR (KBr, cm -1 ) Spectrum: 3392 (-OH), 2969, 2933 (saturated hydrocarbon), 1652, 1647 (C=C), 1456, 1378 (gem dimethyl).

[0136] 2. Determination of molecular formula

[0137] m / z 483.3104[M+Na] given by HR-ESI-MS + (calcd.483.3081, err2.3), it is deduced that the molecular weight of the compound is 460.32. exist 1 H-NMR and 13 C-NMR shows that there are 40 hydrogen signals and 28 carbon signals in total. From DEPT shows that there are 6 quaternary carbons, 10 CH, 6 CH 2 and 6 CH 3 . exist 13 In C-NMR, there are 4 olefinic carbon signals a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of medicine and chemical industry, and relates to an anticancer application of a sterols derivative. The sterols derivative is a compound shown as the formula I, or a pharmaceutically acceptable salt thereof, wherein R1 is selected form -OH, =O, H and C1-C3 alkyl; R2 is selected form -OH, H and C1-C3 alkyl; R3 is selected form -OH, =O, H and C1-C3 alkyl; R4 is selected form -OH, H and C1-C3 alkyl; and any two, three or four of R1, R2, R3 and R4 are -OH simultaneously. The compound provided by the invention is capable of effectively inhibiting proliferation of cancer cells (tumor cells), and the inhibition effect is in concentration-effect relationships, thus the sterols derivative has the potential of a medicament for prevention and / or treatment and / or adjuvant treatment of cancers.

Description

technical field [0001] The invention belongs to the field of medicine and chemical industry, and relates to the anticancer application of sterol derivatives. Background technique [0002] Monascus-fermented rice is a purple-red rice koji made from rice as raw material and fermented by Monascus. Red koji was called Danqu in ancient times, and it was fermented by inoculating koji or distiller's yeast based on Monascus on rice. Its color is red, so it is also called red koji, red rice, red rice, and red rice. Fujian and other places. Therefore, it is also known as Fuqu, Fumi, etc. [0003] my country has a long history of using Monascus, and it has been used to make koji since the Han Dynasty. Red yeast rice is a traditional Chinese medicine that combines both diet and therapy. As early as in ancient times, it has been widely used in food coloring, wine making, fermentation, and traditional Chinese medicine. "Drinking Zheng Yao" has red yeast rice "sweet, flat, non-toxic", ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/58C07J71/00A61P35/00
Inventor 段震文郭树仁李雪梅
Owner BEIJING WBL PEKING UNIV BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com