Screening method for genes related to physical ability of excellent cross-country skiers

A screening method and technique for athletes, applied in biochemical equipment and methods, determination/inspection of microorganisms, etc., and can solve problems such as screening methods that have not yet been discovered

Inactive Publication Date: 2013-06-19
HARBIN INST OF PHYSICAL EDUCATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] According to the literature search, there is currently no method for screening genes related to physical fitness of excellent cross-country skiers using gene chips

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1: Analysis and Screening of Genes Related to Physical Fitness by Gene Chip Analysis of Families of Excellent Cross-country Skiers

[0011] 1. Selection of research objects

[0012] Taking the families of outstanding cross-country skiers as the research object. "Family of excellent cross-country skiers" is defined as at least one of the first-degree relatives is an excellent cross-country skier. Conduct a survey on each outstanding cross-country skier to determine the research subjects, and determine the family members of the first-degree relatives of each outstanding cross-country skier (including parents, siblings and children, regardless of age and gender).

[0013] Healthy adults aged 18 to 75 who had no blood relationship with the excellent cross-country skiers in the test group were used as references to screen the genes related to the physical fitness of the excellent cross-country skiers.

[0014] Each subject must sign an informed consent form.

[00...

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PUM

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Abstract

The invention provides a screening method for the genes related to physical ability of excellent cross-country skiers. The screening method provided by the invention comprises the following steps of: taking an excellent cross-country skier system as an object; comparing the physical ability differential gene expression profiles of the excellent cross-country skiers by applying a high-flux oligonucleotide chip technology; analysing and explaining the molecular functions, signal pathways and cellular localization information of differential genes via gene ontology, thus screening the target pathways related to physical ability heredity; looking for the pathways where the differential genes are located via BioCarta and KEGG website; screening the significant target pathways by applying a hypergeometric distribution statistical method; and verifying the differential genes by real-time fluorescence quantification (RT-PCT), so as to obtain the target genes related to physical ability of the excellent cross-country skiers.

Description

technical field [0001] The invention relates to a molecular biology method for screening genes related to physical fitness of excellent cross-country skiers, specifically, screening genes related to physical fitness of families of excellent cross-country skiers by gene chip method and bioinformatics analysis. It is suitable for the early genetic selection of excellent cross-country skiers and belongs to the interdisciplinary field of sports science, genetics, bioinformatics and molecular biology. Background technique [0002] Cross-country skiing is one of the most popular extreme sports. Cross-country skiing is a sport dominated by physical fitness, and the strength and endurance of athletes play a decisive role in sports performance. Cross-country skiing emphasizes the pleasure and sense of accomplishment obtained when bravely crossing psychological barriers, and also pursues "faster, higher, and stronger" in competitive sports beyond physical limits. Therefore, as one o...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 王旋张强高俊江金红王文杰关伟军
Owner HARBIN INST OF PHYSICAL EDUCATION
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