Method capable of selecting and screening surfactant hydrocarbon degradation bacteria on surface of forming material

Abstract

The invention provides a method capable of rapidly selecting and screening surfactant petroleum hydrocarbon degradation pure bacterial strain from oil soil. According to the method capable of rapidly selecting and screening the surfactant hydrocarbon degradation bacteria on the surface of forming material, the oil soil is collected from an oil field and added into a hydrocarbon degradation culture medium with the amount of 5%, wherein crude oil of the hydrocarbon degradation culture medium is a sole carbon source, the content of the crude oil is gradually improved, and an enrichment culture is carried out on a constant temperature shaker. After the enriched bacteria liquid is diluted to a certain degree and coated to be cultured on a blood plate, 1-3d is cultured at the temperature of 30 DEG C, bacteria colony with a clear and large transparent zone edge on the blood plate is taken, after the bacteria colony is cultured in a liquid meat peptone culture medium, the bacteria liquid is dipped through a sterilizing toothpick blunt end and dibbled on an oil plate, 3d is cultured at the temperature of 30 DEG C, bacteria colony with an obvious and large oil biting spot is taken to be vaccinated in a meat peptone inclined surface and stored, after the bacteria colony is cultured with the meat peptone culture medium, the hydrocarbon degradation culture medium and the surfactant culture medium are vaccinated in sequence, 7d and 3d are cultured at the temperature of 30 DEG C, the hydrocarbon degradation capacity and the surfactant capacity are determined, and therefore the surfactant petroleum hydrocarbon degradation bacterial strain can be obtained.

Description

technical field [0001] The invention relates to a method for separating and screening hydrocarbon-degrading bacteria producing surfactant, and belongs to the fields of environmental bioengineering and environmental microorganisms. Background technique [0002] With the exploitation, transportation and leakage of oil and the discharge of oily wastewater, more than 8 million tons of oil enter the environment every year, polluting soil, groundwater, rivers and oceans, causing great harm to the ecological environment and affecting production and people's lives. Therefore, the problem of oil pollution has attracted more and more attention, and its governance has become the most urgent thing. [0003] There are three methods of remediation of oil-contaminated environments: physical, chemical and biological remediation. Microbial remediation technology is the most important and core component of bioremediation. It has the advantages of low cost, simple application method, complete...

AI Technical Summary

Problems solved by technology

Although the production of surfactants is considered to be one of the mechanisms by which microorganisms emulsify petroleum to promote degradation, the method of screening hydrocarbon-degrading bacteria producing surfactants, especially the high-efficiency primary screening method, still makes researchers feel difficult

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