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Method for producing diosgenin through microbial fermentation of peltate yam rhizome-yellow ginger

A technology of microbial fermentation and diosgenin is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of reducing the amount of wastewater generated, high efficiency, and reducing conversion costs.

Active Publication Date: 2014-07-09
西安绿泉科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the above-mentioned shortcoming of the prior art and solve the bottleneck problem of producing diosgenin by microbial transformation, the present invention aims to provide a method for producing diosgenin by microbial transformation from Dioscorea scutellariae-Turmeric

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Preparation of seed solution

[0019] Take 5ml of bacteria with a density of 10 8 ~10 9 / ml Bacillus aryabhattaiThe XBT2011 suspension was inoculated into 150ml seed culture solution, and cultured at 40°C, 200rpm for 14hrs to obtain the seed solution. Wherein the seed culture solution is composed of the following components according to the following mass volume percentages: turmeric dry powder 5%, urea 0.3%, yeast extract 0.1%, N a Cl 0.1%, MgSO 4 0.05%, k 2 HPO 4 0.05%, FeSO 4 0.005%, CaCl 2 0.001%.

[0020] Preparation of conversion slurry

[0021] Add 500g turmeric dry powder and 3000mL tap water to a 5L fermenter, add 20g urea, 5.0g yeast extract, N a Cl 3.0g, MgSO 4 1.5g, k 2 HPO 4 1.5g, FeSO 4 0.15g, CaCl 2 0.03g ingredients, of which the inorganic salt is 0.2% of the volume of tap water, and the pH is adjusted to 7.0±0.2. Sterilize at 121°C and 0.1Mpa pressure for 15 minutes (minutes).

[0022] Transformation of diosgenin

[0023] ...

Embodiment ２

[0030] Basically the same as Example 1, the difference is:

[0031] Add 600g turmeric dry powder and 3000mL tap water to a 5L fermenter, add 25g urea, 6.0g yeast extract, N a Cl 3.0g, MgSO 4 1.5g, k 2 HPO 4 1.5g, FeSO 4 0.15g, CaCl 2 The components such as 0.03g are converted into slurry, wherein the inorganic salt is 0.2% by volume of tap water. Insert 200ml of XBT2011 strain seed solution cultured for 14hrs into the sterilized transformation slurry, and transform for 72hrs at 40°C, 400rpm, 3.5 l / min, and pH 6.0. The conversion slurry was filtered and dried to finally obtain a hydrolyzate with a dry weight of 198.7g, which was 33.1% of the dry weight of the added turmeric dry powder. The hydrolyzate was extracted with one volume of 120﹟ gasoline for 12-16hrs, and industrial activated carbon was added to remove the pigment. After repeated crystallization, 16.5g of finished diosgenin was obtained, and the yield of diosgenin was 2.7%. The diosgenin was determined by RP...

Embodiment 3

[0033] Basically the same as Example 12, the difference is:

[0034] Add 250g dry turmeric powder and 3000mL water to a 5L fermenter, add 10g urea, 2.5g yeast extract, N a Cl 3.0g, MgSO 4 1.5g, k 2 HPO 4 1.5g, FeSO 4 0.15g, CaCl 2 The components such as 0.03g are converted into slurry, wherein the inorganic salt is 0.2% by volume of tap water. Into the sterilized transformation slurry, insert 200ml of XBT2011 strain seed solution cultivated for 14hrs, and transform for 56hrs at 40°C, 300rpm, 2.5 l / min, and control pH 6.2. The culture slurry was filtered and dried to obtain a hydrolyzate with a dry weight of 70.5g, which was 28.2% of the dry weight of the added turmeric dry powder. The hydrolyzate was extracted with one volume of 120﹟ gasoline for 12-16hrs, and industrial activated carbon was added to remove the pigment. After crystallization, 7.63g of finished diosgenin was obtained, and the yield of diosgenin was 3.05%. The diosgenin was determined by RP-HPLC The c...

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Abstract

The invention discloses a method for producing diosgenin through microbial conversion of peltate yam rhizome-yellow ginger. Yellow ginger dry powder and tap water are mixed according to a massic volume ratio of 1:5-12, urea which is 4.0% of mass of the yellow ginger dry powder, yeast extract which is 1.0% of mass of the yellow ginger dry powder and inorganic salt which is 0.2% of volume of the tap water are supplemented to prepare conversion slurry, the conversion slurry is introduced in bacillus bacterial strain XBT 2011 seed liquid which is 5% of the volume of the tap water, under the condition that the culture temperature is 40 DEG C, the pH is controlled to be about 6.0, the stirring speed is 300-400rpm and the ventilation condition is 2.5-4.0l / min, the conversion is 56-72hrs, conversion slurry is filtered and dried to obtain hydrolysate, the hydrolysate is extracted for 12-16hrs by using 120# gasoline, the volume of the 120# gasoline is one time of that of the hydrolysate, and diosgenin end products are obtained through repeated crystallization. A preservation number of the bacterial strain XBT 2011 is CGMCCNo.6301. The dry weight of the hydrolysate is 28-33% of that of the added yellow ginger dry powder, total saponins of the peltate yam rhizome is approximately converted into diosgenin completely, handling capacity is big, no sugar contains, the pH is close to neutral, and environmental pollution is reduced.

Description

technical field [0001] The invention relates to a method for preparing diosgenin, in particular to a method for producing diosgenin from dioscia scutellaria-turmeric through microbial transformation. It belongs to the technical field of biological extraction. Background technique [0002] The most commonly used method to extract diosgenin (saponin) from turmeric is acid hydrolysis. This earliest production process has incomplete hydrolysis, low yield (yield is generally only about 1.7%), huge amount of waste water and serious environmental pollution and many other issues. At present, various enterprises adopt the method of coupling the improved natural fermentation and acid hydrolysis. Natural fermentation, through the enzymatic hydrolysis of turmeric's own endogenous enzymes, converts a part of furostyl alcohol saponin into spiroginyl saponin, which is more easily converted into diosgenin. Therefore, this coupling method produces saponin, and its yield can be increased ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/20C12R1/07
Inventor 熊本涛
Owner 西安绿泉科技有限公司
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