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Production method for morchella importuna cultivars

A kind of technology of morel and its production method, which is applied in the fields of botany equipment and method, horticulture, application, etc., and can solve the problems of long production cycle, easy aging of hyphae growth, and low production of conidia, etc.

Inactive Publication Date: 2013-06-12
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the production period of Morchella genus fungi is long (35 days at the shortest) and the mycelium growth is easy to age (such as the invention patent of application number 201010185127.3 applied by Cheng Yuanhui, etc.), the amount of conidia produced after sowing is small, and the fruiting is not good. Stablize

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Preparation of Morchella typhimurium strains: use conventional edible fungus strain preparation and cultivation methods to make the original seed of Morchella typhimurium, cultivated at 18°C ​​for 3 days.

[0018] 2. Proportion of cultivation material: The formula of cultivation material is 80% wheat grain, 18% soil, 1% lime, and 1% superphosphate in a glass bottle.

[0019] 3. Bagging and sterilization: Stir the above ingredients thoroughly, add water to 65% of its water content, put it into a glass bottle, and sterilize at 121°C for 2 hours.

[0020] 4. Vaccination: Routine vaccination.

[0021] 5. Cultivation: Incubate the inoculated glass bottles at 18°C ​​in the dark for 20 days.

Embodiment 2

[0023] 1. Preparation of Morchella typhimurium strains: use conventional edible fungus strain preparation and cultivation methods to make the original seed of Morchella typhimurium, cultivated at 18°C ​​for 3 days.

[0024] 2. Proportion of cultivation material: The formula of cultivation material is 85% wheat grain, 13% soil, 1% lime, and 1% superphosphate in a glass bottle.

[0025] 3. Bagging and sterilization: Stir the above ingredients thoroughly, add water to 65% of the water content, put it into a glass bottle, and sterilize at 125°C for 2 hours.

[0026] 4. Vaccination: Routine vaccination.

[0027] 5. Cultivation: Incubate the inoculated glass bottles at 20°C in the dark for 15 days.

Embodiment 3

[0029] 1. Preparation of Morchella typhimurium strains: use conventional edible fungus strain production and cultivation methods to make the original seed of Morchella typhimurium, which is cultivated at 20°C for 5 days.

[0030] 2. Proportion of cultivation material: The formula of cultivation material is 90% wheat grain, 28% soil, 1.5% lime, and 0.5% superphosphate in a glass bottle.

[0031] 3. Bagging and sterilization: Stir the above ingredients thoroughly, add water to 60% of the water content, put it into a glass bottle, and sterilize at 100°C for 20 hours.

[0032] 4. Vaccination: Routine vaccination.

[0033] 5. Cultivation: Culture the inoculated glass bottles at 23°C in the dark for 18 days.

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Abstract

The invention discloses a production method for morchella importuna cultivars. The production method comprises the following steps of: routinely inoculating morchella importuna stock seeds which are cultured at 18 to 20 DEG C for 3 to 5 days to a glass bottle containing a culture material which comprises 70 to 90 percent of wheat, 8 to 28 percent of soil, 1 to 1.5 percent of lime and 0.5 to 1 percent of calcium superphosphate, and culturing the morchella importuna stock seeds at 18 to 23 DEG C for 15 to 20 days under a dark condition to obtain the morchella importuna cultivars. According to the method, the activity of morchella importuna hyphae can be kept, and sclerotia can be quickly produced; and a production process is simple, and conidia are high in yield and easy to seed.

Description

Technical field [0001] The present invention belongs to the field of biotechnology, and in particular relates to a method for preparing the cultivated species of Morchella edulis. Background technique [0002] Morchella importuna M. Kuo, O’Donnell & T.J. Volk, Asomycotina, Discomycetes, Pezizales, Morchellaceae, Morchella, Morchella. Mainly distributed in my country's Yunnan, Sichuan, Shaanxi, California, Nevada, Northwest Oregon and British Columbia in Canada. The fruit body is rich in amino acids and vitamins, the meat is crisp and tender, and the flavor is unique. It is one of the treasures in edible and medicinal fungi. [0003] Cultivation production is the main work in the early stage of edible fungus cultivation. Although the production process and technical requirements are basically the same, different culture material formulas play a key role in the quality of cultivars. At present, the fungus species of the genus Morchella has a long production cycle (the shortest 35 d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 赵琪杨祝良赵永昌
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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