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Streptomyces YT027 and application thereof

A technology of YT027 and Streptomyces, applied in the application, bacteria, food preservation and other directions, can solve the problem of no taro blight and other problems, and achieve the effect of significant biocontrol effect, prolonged storage period and high antagonistic activity.

Active Publication Date: 2013-06-05
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of Streptomyces to control taro blight, including taro blight during storage.

Method used

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  • Streptomyces YT027 and application thereof
  • Streptomyces YT027 and application thereof
  • Streptomyces YT027 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Isolation and Identification of Streptomyces YT027

[0031] 1) Separation of bacteria resources to be screened: Scrape 100 g of the adhered soil on the surface of healthy taro, mix well, take 10 g of soil and pour it into a 250 mL conical tube containing 20 fine glass beads and 100 mL of sterilized deionized water. The flasks were placed on a shaker at 150 r / min and shaken at 30°C for 6 h. After standing still for 10 min, take 1 mL of the supernatant, dilute it 10 times with sterilized deionized water, draw 100 μL of each concentration gradient dilution, apply it to a 2YT solid plate, and culture at 30°C for 36 h. A single colony was picked with an inoculation loop, streaked and purified twice on 2YT solid medium, and the numbers of purified single colonies of different forms were transferred to a 2YT solid plate, cultured at 30°C for 36 h, and stored at 4°C.

[0032]2) Plate antagonism screening of YT027: The purified strains stored on 2YT solid plat...

Embodiment 2

[0035] Embodiment 2 utilizes streptomyces YT027 to prepare the method for biocontrol agent

[0036] Take the bacterial classification of Streptomyces strain YT027 of the present invention, streak and inoculate it on a fresh 2YT solid plate, and activate and cultivate it at 25°C for 36 h; In a mL Erlenmeyer flask, cultured for 36 h at an oscillation frequency of 150 r / min and 30 °C as a seed solution; the seed solution was inoculated in the fermentation medium at a ratio of 1% (v / v) for fermentation, and the fermentation The temperature was 30°C, the fermentation was aerated with an aeration ratio of 0.2:1, the stirring speed was 120 r / min, and the cultivation was carried out for 36 h.

[0037] The fermentation medium is: 10 g soluble starch, 15 g soybean meal powder, 0.5 g NaCl, KH 2 PO 4 0.5 g, MgSO 4 ·7H 2 O 0.5 g, FeSO 4 ·7H 2 O 0.16 mg, ZnSO 4 ·7H 2 O 0.16 mg, CuSO 4 ·5H 2 O 0.16 mg, MnSO 4 5 mg, distilled water to 1000 mL, pH 6.3, sterilized at 121°C for ...

Embodiment 3

[0038] Embodiment 3 utilizes streptomyces YT027 to prepare the method for biocontrol agent

[0039] Take the bacterial classification of Streptomyces strain YT027 of the present invention, streak and inoculate it on a fresh 2YT solid plate, and activate and cultivate it at 32°C for 24 h; In a mL Erlenmeyer flask, cultured for 48 h under the condition of shaking frequency of 100 r / min and 25 °C, as the seed solution; the seed solution was inoculated in the fermentation medium at a ratio of 10% (v / v) for fermentation, and the fermentation The temperature was 25°C, the fermentation was aerated with an aeration ratio of 2.5:1, the stirring speed was 75r / min, and the cultivation was carried out for 48 h.

[0040] The fermentation medium is: glucose 5 g, yeast extract 10 g, NaCl 2 g, KH 2 PO 4 1 g, MgSO 4 ·7H 2 O 1 g, FeSO 4 ·7H 2 O 0.16 mg, ZnSO 4 ·7H 2 O 0.16 mg, CuSO 4 ·5H 2 O 0.16 mg, MnSO 4 5 mg, dilute to 1000 mL with distilled water, pH 7.5, and sterilize at ...

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Abstract

The invention provides a streptomyces sp. strain YT027 which is preserved in China General Microbiological Culture Collection Center with the address of 3#, No.1 Yard, Beichen West Road, Chaoyang District, Beijing (100101) on January 23rd, 2013, and the preservation number is CGMCC No. 7196. The streptomyces YT027 provided by the invention has higher antagonistic activity to Phytophthora colocasiae Racib., the fermentation culture liquid of the streptomyces YT027 has good biocontrol effects on sweet potato diseases in the preservation period, can avoid or reduce chemical pesticide residue, remarkably prolong the storage period of sweet potatoes and maintain the quality of the sweet potatoes, and has better economic and social benefits.

Description

technical field [0001] The invention belongs to the technical field of storage and preservation of agricultural products, and in particular relates to a Streptomyces strain YT027 and its application, which are specially used for pollution-free biological control of taro blight during storage. Background technique [0002] Taro blight is caused by Phytophthora taro ( Phytophthora colocasiae Racib.) caused fungal diseases of taro. Although taro blight is one of the main diseases during the cultivation period of taro, a large number of wounds are produced when taro is harvested, and the ambient humidity in the early storage period is relatively high, breathing is vigorous, and the temperature is high. It is easy to infect and disease, causing deterioration and rot of taro during storage. Especially its mixed attack with bacterial soft rot, which is more harmful. [0003] The effective control of taro blight during storage is an important aspect to prolong the storage period...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23B7/155C12R1/465
Inventor 赵延存李鹏霞黄开红王毓宁
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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