High-valued utilization method for patinopecten yessoensis offal
A technology for shrimp scallops and leftovers, which is applied in the field of high-value utilization of shrimps and scallops, can solve the problems of low utilization rate of leftovers, waste of resources, etc., and achieves high added value of products, shortened time, and mild reaction conditions. Effect
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example 1
[0037] ⑴Raw material processing: After washing the scallop scraps, add 2 times the weight of distilled water to homogenate to make a homogenate;
[0038] (2) Enzymolysis: Add trypsin and Bacillus subtilis neutral protease to the above homogenate, the amount of enzyme added is 0.8% of the total weight of the waste, and trypsin and Bacillus subtilis neutral protease each account for half. Enzymolysis for 3.5 hours; separate the supernatant: boil the enzymolysis solution to kill the enzyme, centrifuge at 5000r / min for 10min, separate the supernatant and precipitate;
[0039] (3) Decolorization: add 5% hydrogen peroxide to the supernatant to decolorize;
[0040] (4) Remove protein: first adjust the pH to 2.0 with HCl, and remove the precipitate by centrifugation; then adjust the pH to 7.0 with NaOH, and remove the precipitate by centrifugation;
[0041] (5) Alcohol precipitation method to extract crude glycosaminoglycans: slowly add ethanol to the supernatant under constant stirr...
example 2
[0048] ⑴Raw material processing: After washing the scallop scraps, add 3 times the weight of distilled water to homogenate to make a homogenate;
[0049] (2) Enzymolysis: Add trypsin and Bacillus subtilis neutral protease to the above homogenate, the amount of enzyme added is 1% of the total weight of the waste, and trypsin and Bacillus subtilis neutral protease account for half each. Enzymolysis for 4 hours; separation and supernatant: after boiling the enzymolysis solution to kill the enzyme, centrifuge at 5000r / min for 10min, and separate the supernatant and precipitate;
[0050] (3) Decolorization: add 7% hydrogen peroxide to the supernatant to decolorize;
[0051] (4) Remove protein: first adjust the pH to 2.0 with HCl, and remove the precipitate by centrifugation; then adjust the pH to 7.0 with NaOH, and remove the precipitate by centrifugation;
[0052] ⑸ Alcohol precipitation method to extract crude glycosaminoglycans: slowly add ethanol to the supernatant under const...
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