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Construction for number 1 chromosome substitution laboratory mouse strain C57BL/6-Chr1AKR

A technology of chromosome replacement and chromosome, applied in animal husbandry and other fields

Inactive Publication Date: 2013-06-05
上海西普尔-必凯实验动物有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to technical difficulties in the construction of chromosome replacement strains, the inbred line AKR has not been introduced into the genome of the recipient strain C57BL / 6 mice

Method used

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  • Construction for number 1 chromosome substitution laboratory mouse strain C57BL/6-Chr1AKR
  • Construction for number 1 chromosome substitution laboratory mouse strain C57BL/6-Chr1AKR
  • Construction for number 1 chromosome substitution laboratory mouse strain C57BL/6-Chr1AKR

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] Embodiment 1, the construction method of chromosome replacement mouse

[0103] Adult mice of two strains of AKR and C57BL / 6 were taken, and AKR was used as the female parent, and C57BL / 6 was used as the male parent to cross each other to obtain the F1 generation.

[0104] C57BL / 6 was obtained by crossing F1 generation male mice with C57BL / 6 female mice 1 -Chr1 AKR , for backcross generation, in C57BL / 6 1 -Chr1 AKR In male mice, the sex chromosomes and mitochondrial DNA in AKR have been replaced with C57BL / 6; F2 generation male mice were crossed with C57BL / 6 female mice to obtain C57BL / 6 2 -Chr1 AKR , in this way, ten generations of recurrent crosses were obtained, and ten generations of backcrossed mice C57BL / 6 were obtained. 10 -Chr1 AKR , the schematic diagram of the construction process is as follows figure 1 .

Embodiment 2、1

[0105] Example 2, No. 1 chromosome genome identification

[0106] (1) Primary Screening and Identification of Chromosome 1 Genome

[0107] Compare the gene sequences of chromosome 1 of the two strains of AKR and C57BL / 6 (refer to the public information of MGI (http: / / www.informatics.jax.org / ) for the sequence), and select the representative two strains Different SNP sites, these SNP sites are evenly distributed on chromosome 1, and are easy to design and operate. Then design primers for these SNP sites to amplify sequences with characteristic SNP sites, and design the range of PCR amplified fragments between 100 and 400 bp. Primer design is mainly based on primer3 online software (http: / / frodo.wi .mit.edu / primer3 / ) and the Oligo6.0 program (Molecular Biology Insights Inc., USA) were completed. Site-specific probes and general probes were designed for these SNP sites for LDR, and the length of LDR probe ligation products was 80-130 bp.

[0108] The 13 SNP sites on the chromo...

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Abstract

The invention relates to construction for a number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1AKR. According to the construction for the number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1AKR, a new chromosome substitution laboratory mouse strain is constructed. A genetic background of the chromosome substitution laboratory mouse strain is based on a C57BL / 6 mouse, while wherein a number 1 chromosome is from an AKR mouse. According to the construction for the number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1AKR, the chromosome substitution laboratory mouse strain can be used for research of character differences brought by different number 1 chromosome genomes under the same genome background, and can be a useful tool for research of the chromosome genome functions by adoption of a genetic method.

Description

technical field [0001] The invention belongs to the field of mouse strain construction, in particular relates to the construction of a No. 1 chromosome replacement experimental mouse strain C57BL / 6-Chr1 AKR . Background technique [0002] Existing inbred strains of mice have a single ancestor, and the number of alleles at the same gene locus is small, but the alleles that cause differences in complex traits in natural populations are very rich. At present, the classic method for locating genes related to complex traits in mice is Crossing different mouse strains with specific trait differences and initially mapping QTLs generally takes about 6 months, and the length of the mapping segment is about 40-cM. However, it will take several years to construct congenic lines and carry out fine mapping of genes to narrow down the QTL segment to 1cM (including about 10-20 genes on average). Further narrowing the QTL segment requires repeated backcrossing or selfing to generate chrom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027
Inventor 赵莹赵丽亚刘磊邢正弘陈国强肖君华
Owner 上海西普尔-必凯实验动物有限公司
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