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Atomic force microscope (AFM) probe rapid positioning method for cell mechanical property detection

A technology of mechanical characteristics and positioning method, applied in the field of nanometer operation, can solve problems such as inability to meet measurement requirements, low efficiency, time-consuming, etc., and achieve the effect of improving probe operation efficiency, improving accuracy, and achieving accurate calibration

Inactive Publication Date: 2015-07-08
SHENYANG INST OF AUTOMATION - CHINESE ACAD OF SCI
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Problems solved by technology

Since the AFM imaging speed is extremely slow and often takes several minutes, this method of scanning the imaging-manually moving the probe to achieve the positioning of the AFM needle tip and the center of the cell to be tested is very time-consuming and inefficient, and obviously cannot meet the needs of large quantities. Measuring needs for mechanical properties of cells

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  • Atomic force microscope (AFM) probe rapid positioning method for cell mechanical property detection
  • Atomic force microscope (AFM) probe rapid positioning method for cell mechanical property detection

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Embodiment Construction

[0028] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0029] Fast local scanning algorithm: the probe tip is located under the cantilever beam, and its real position cannot be observed. Optical images can only be used to identify the relative positional relationship between the probe cantilever beam and the cell. The relative positional relationship between the needle tip and the cell.

[0030] Such as figure 1 As shown, the specific process of performing partial scanning is: the actual cell center can be determined by two lines - a horizontal scanning line and a vertical scanning line. Using the actual distance between the cell and the probe cantilever, position the probe to the left of the cell, and then scan the cell along the horizontal direction, we can get two intersection points between the horizontal scanning line L and the edge of the cell P and Q, then the vertical scan line V can be ...

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Abstract

The invention relates to the technical field of nanometer operation, particularly to an AFM probe rapid positioning method for cell mechanical property detection. The method includes recognizing cells through carrying out a Hough transform round detection mode in cell edge images, obtaining radiuses and central position information of each cell to be detected simultaneously, and calculating the actual distance between each cell to be detected and a probe cantilever beam in working space; determining the relative position relation between the probe tip and cells to be detected through rapid local scanning of cells to be detected; and sequentially achieving rapid positioning of the AFM probe to measuring points of cells to be detected to complete measurement of the mechanical property of each cell. According to the rapid positioning method, a visual image processing technology is used for calibrating the relative position relation between the cells to be detected and the probe cantilever beam in the working space, programming control from probe motion to cells to be detected can be achieved, and the operating efficiency of the probe is improved; and the rapid local scanning method is used, so that accurate calibration of the AFM tip and the cell relative positions is achieved, and the accuracy of cell mechanical property measurement is improved.

Description

technical field [0001] The invention relates to the technical field of nano-manipulation, and more specifically relates to a rapid positioning method of AFM probes for the detection of cell mechanical properties. Background technique [0002] The measurement of the mechanical properties of living cells is a hot direction of AFM (Atomic Force Microscope, atomic force microscope) in biological applications. The mechanical properties of cells play an important role in key cellular activities (migration, division, deformation, etc.). In the past 10 years, studies of the mechanical properties of individual cells have shown that the mechanical function of cells is closely related to the physical condition of humans. Changes in cell mechanical properties may be a precursor to certain diseases, and diseases themselves may also lead to changes in cell structure and mechanical properties. Therefore, cell mechanical properties have recently been considered as a new biomarker. It help...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01Q10/00
CPCG01Q30/04G01Q60/42
Inventor 王越超刘连庆王智博董再励袁帅张常麟
Owner SHENYANG INST OF AUTOMATION - CHINESE ACAD OF SCI
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