Fluorescent PCR (polymerase chain reaction) quick detection primer and kit for Xanthomonas campestris pv. campestris

Abstract

The invention relates to a real-time fluorescent PCR (polymerase chain reaction) quick detection primer and kit for Xanthomonas campestris pv. campestris. The primer comprises a forward primer of which the sequence is SEQ ID NO:1 and a reverse primer of which the sequence is SEQ ID NO:2. In the invention, a specific conserved sequence is obtained through molecular biological analysis according to a pthBXam and Tn3 gene sequence of Xanthomonas campestris pv. manihotis, and a specific amplification primer thereof is designed. The conserved gene sequence is shared by different Xanthomonas campestris pv. manihotis strains to ensure the reliability in detection of Xanthomonas campestris pv. manihotis from different sources on a species level. Besides, the primer provided by the invention adopts fluorescence labeling; and compared with the common PCR technology, observation does not need to be performed through a gel electrophoresis method, thus realizing the integrated sealed detection in the detection process.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a fluorescent PCR rapid detection primer for Xanthomonas rape pathogenic species and a kit containing the primer. Background technique [0002] Xanthomonas rapeusae pv. cassava is a pathogenic variety of Xanthomonas oleracea. It is the most serious infection disease of cassava crops. Cassava production has caused irreparable losses many times, and with the introduction of foreign high-quality varieties, along with the colonization and breeding promotion of these propagation materials, it has spread and invaded my country. And gradually expand the scope of harm, the trend of harm is intensified, and the consequences of harm are becoming more serious. At present, in my country's major cassava producing areas such as Guangxi, Guangdong, and Hainan, driven by the inherent tropical storms in this area, and under the action of environmental factors such as unique terrain, culti...

AI Technical Summary

Problems solved by technology

However, in the process of detection, ELISA detection showed serious limitations: the results of some susceptible stem tissues were false negative, some cassava bacterial wilt strains did not undergo enzyme-linked reactions, and some Xanthomonas pathogenic Mutant pathogens such as citri, euphorbiae, and vasculorum cross-react

These limitations not only hinder the study of the ultimate limit of ELISA detection, but also greatly limit its application

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