Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Electrochemical sensor for mercury ion detection, and manufacturing method and detection method thereof

A manufacturing method and sensor technology are applied in the field of biological analysis to achieve the effects of simple manufacturing method, high specificity and simple steps

Active Publication Date: 2013-05-15
CAPITAL NORMAL UNIVERSITY
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the development of Hg with extremely high sensitivity and selectivity and a sufficiently wide kinetic range 2+ The detection method is still a challenge to the majority of researchers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Electrochemical sensor for mercury ion detection, and manufacturing method and detection method thereof
  • Electrochemical sensor for mercury ion detection, and manufacturing method and detection method thereof
  • Electrochemical sensor for mercury ion detection, and manufacturing method and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Construction of Hg when the whole thymine (T) oligonucleotide chain assembled on the surface of the gold electrode is low density 2+ Detection of electrochemical sensors.

[0038] Soak an unmodified clean gold electrode in 10 mM Tris, 0.2 M NaCl, pH=8.0 containing 1 μM probe 1 that has been reduced with tris[2-carboxyethyl]phosphine (TCEP) for 1 h, and place it at room temperature for 1 h . The assembled electrode was rinsed twice with 10mM Tris, 0.5M NaCl, pH = 8.0, then placed in an aqueous solution containing 1mM mercaptohexanol (MCH) to block for 30 minutes, and then washed with 10mM Tris, 0.5M NaCl, pH = 8.0 Wash three times, then react with 500nM probe 2 in 1 / 15M PB, 0.3M NaCl, pH=7.4 solution for 2h, 25°C. Then wash the electrode with 1 / 15M PB, 0.1M NaCl, pH=7.4 solution for three times and store it at 4°C for future use.

Embodiment 2

[0039] Example 2: Construction of Hg when the whole thymine (T) oligonucleotide chain assembled on the surface of the gold electrode is medium density 2+ Detection of electrochemical sensors.

[0040] Soak an unmodified clean gold electrode in 10 mM Tris containing 1 μM probe 1 that has been reduced with tris[2-carboxyethyl]phosphine (TCEP) for 1 h, 1.0 M NaCl, pH=8.0, and place at room temperature, Self-assemble overnight. The assembled electrode was rinsed twice with 10mM Tris, 0.5M NaCl, pH = 8.0, then placed in an aqueous solution containing 1mM mercaptohexanol (MCH) to block for 30 minutes, and then washed with 10mM Tris, 0.5M NaCl, pH = 8.0 Wash three times, then react with 500nM probe 2 in 1 / 15M PB, 0.3M NaCl, pH=7.4 solution for 2h, 25°C. Then wash the electrode with 1 / 15M PB, 0.1M NaCl, pH=7.4 solution for three times and store it at 4°C for future use.

Embodiment 3

[0041] Example 3: Construction of Hg when the whole thymine (T) oligonucleotide chain assembled on the surface of the gold electrode is at high density 2+ Detection of electrochemical sensors.

[0042] Soak an unmodified clean gold electrode in 100mM PB containing 1μM probe 1 that has been reduced with tris[2-carboxyethyl]phosphine (TCEP) for 1h, 1.5M NaCl, pH=7.2, and place at room temperature, Self-assemble overnight. The assembled electrode was rinsed twice with 10mM Tris, 0.5M NaCl, pH = 8.0, then placed in an aqueous solution containing 1mM mercaptohexanol (MCH) to block for 30 minutes, and then washed with 10mM Tris, 0.5M NaCl, pH = 8.0 Wash three times, then react with 500nM probe 2 in 1 / 15M PB, 0.3M NaCl, pH=7.4 solution for 2h, 25°C. Then wash the electrode three times with 1 / 15M PB, 0.1M NaCl, pH=7.4 solution, and store it at 4°C for future use.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an electrochemical sensor for mercury ion detection, and a manufacturing method and a detection method thereof and belongs to the technical field of bioanalysis. The electrochemical sensor is a gold electrode, wherein the gold electrode comprises a full-thymine (T) oligonucleotide chain which is fixed on the surface of the gold electrode and is subjected to chemical modification, and a ferrocene-marked full-adenine (A) complementary chain hybridized with the full-thymine (T) oligonucleotide chain. The Hg<2+> amount which can be contained by the sensor is be adjusted, so that the dynamic range of the sensor is directly related with the density of the full-thymine (T) oligonucleotide chain on the surface of the gold electrode. When the density of the full-thymine (T) oligonucleotide chains on the surface of the gold electrode are respectively low, medium and high, the corresponding dynamic ranges of the sensor are sequentially 10pM-100nM, 10pM-100uM and 1uM-1mM. The electrochemical sensor assembled by uisng the method has high ion selectivity and can not be interfered by other common metal ions.

Description

technical field [0001] The invention relates to an electrochemical sensor for mercury ion detection, a manufacturing method and a detection method, and belongs to the technical field of biological analysis. Background technique [0002] The dynamic range of an analytical detection method is an extremely important parameter in quantitative analysis. Sensors with a wide kinetic range are especially desirable for on-site detection, where the analyte may exist over a large and unknown concentration range. A large number of studies tend to focus more on improving the sensitivity of the detection method, but few studies on improving the detection kinetic interval. Mercury is a highly toxic global environmental pollutant. Even a very small amount exists in the environment, which poses a great threat to the health of animals, plants and humans. Water-soluble divalent mercury ion (Hg 2+ ) is one of the most common and stable forms of mercury pollution. At present, the traditional...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 娄新徽赵滔
Owner CAPITAL NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com