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Plasmodium infected erythrocyte identification method and device thereof

A technology for plasmodium infection and red blood cells, which is applied in the medical field, can solve problems such as low efficiency and poor recognition accuracy of red blood cells, and achieve the effects of fast inspection, high recognition accuracy, and improved recognition accuracy

Active Publication Date: 2013-05-08
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the embodiments of the present invention is to provide a method for identifying red blood cells infected by malaria parasites, aiming to solve the problems of poor recognition accuracy and low efficiency of red blood cells infected by malaria parasites in existing technical solutions

Method used

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  • Plasmodium infected erythrocyte identification method and device thereof
  • Plasmodium infected erythrocyte identification method and device thereof
  • Plasmodium infected erythrocyte identification method and device thereof

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Embodiment 1

[0097] This embodiment provides a method for identifying red blood cells infected by Plasmodium. The specific technical scenario implemented in this embodiment can be as follows: the method provided in this embodiment is completed by a flow cytometer, and the analyzer can specifically adopt Shenzhen Mindray Biomedical Technology Co., Ltd. The BC series flow cytometer produced by Electronics Co., Ltd., the specific structure of the analyzer is as follows: Figure 7 As shown, the formula of the hemolytic agent can be: reagent A, and the reagent A can specifically include: dye A (0.5ppm), decyl isoquinoline bromide (0.4g / L), polyoxyethylene lauryl alcohol (23) Ether (1.3g / L), sodium benzoate (2.0g / L), methanol (50g / L), sodium dihydrogen phosphate (3g / L) and disodium hydrogen phosphate (4.8g / L). Wherein the structural formula of dye A is as follows:

[0098]

[0099] In addition, the volume of the above-mentioned reagents can be 1 ml, the above-mentioned blood sample can be 20...

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Abstract

The invention is suitable for the medical field, and provides a plasmodium infected erythrocyte identification method and a device thereof. The method comprises the following steps: acquiring the forward diffusion light signal and the lateral diffusion light signal of cells in a sample, and an optional fluorescence signal; obtaining a first two-dimensional point diagram according to the forward diffusion light signal and the lateral diffusion light signal, or obtaining a three-dimensional point diagram based on the forward diffusion light signal, the lateral diffusion light signal and the fluorescence signal; and identifying cells appearing in the preset area of the first two-dimensional point diagram or the three-dimensional point diagram as plasmodium infected erythrocytes. The method has a high identification precision.

Description

technical field [0001] The invention belongs to the medical field, and in particular relates to a technology for identifying red blood cells infected by malaria parasites. Background technique [0002] Plasmodium infection is a common infectious disease in many parts of the world, especially in Africa. Plasmodium enters the human blood circulation system through mosquito bites, and reproduces and proliferates in red blood cells. Malaria often causes clinical symptoms such as fever and chills, and if not treated in time, it may even lead to death. Malaria is an infectious disease that seriously threatens human health. [0003] The current diagnosis of malaria requires comprehensive medical history, travel history, clinical symptoms and microscopic examination results, and the identification of plasmodium-infected red blood cells plays a key role in the identification of malaria. Examination is used to determine the red blood cells infected by malaria parasites, but the exi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/47G01N21/64
CPCG01N33/80G01N2333/445G01N21/64G01N33/5091G01N21/49G01N2021/6493G01N33/56905G01N21/51G01N2021/4707G01N2021/4711G01N2021/4726Y02A50/30G01N33/5094G01N21/47G01N21/6486G01N15/01G01N2015/011G01N2015/012G01N2015/016
Inventor 叶波钱程祁欢
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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