Ultrasonic extraction method of red glandular honeysuckle leaf chlorogenic acid

Abstract

The invention discloses an ultrasonic extraction method of red glandular honeysuckle leaf chlorogenic acid. The method comprises a step of acquiring an ultrasonic extraction method and technological parameters of the chlorogenic acid by using red glandular honeysuckle leaves as raw materials, so that a certain reference function is provided for development and utilization of red glandular honeysuckle leaf resources and industrial production of the chlorogenic acid in the future. The analysis of an experiment result shows that an ultrasonic extraction optimal process condition of chlorogenic acid in the red glandular honeysuckle leaf is as follows: the optimal process condition is A2B2C3D1, i.e. the extraction temperature is 50 DEG C, the ethanol concentration is 60 percent, the extraction is performed by three times, and the extraction feed liquid proportion is eight times. The document reports that the yield of chlorogenic acid extracted from the red glandular honeysuckle leaf by using other methods is between 1 and 2 percent, and the extraction rate of the chlorogenic acid extracted from the red glandular honeysuckle leaf by using the ultrasonic extraction method can be up to more than 1.7 percent. Compared with the traditional method, the ultrasonic extraction method is high in yield, short in extraction time and mild in condition; and therefore, the ultrasonic extraction method is a good extraction method.

Description

technical field [0001] The invention belongs to the field of extracting chlorogenic acid from honeysuckle leaves, in particular to an ultrasonic extraction method for chlorogenic acid from honeysuckle leaves. Background technique [0002] Lonicera red glandularis [1, 2] is the dry flower bud or flower with early blooming of Lonicera hypoglauca Miq., a plant of the Lonicera family Lonicera hypoglauca Miq. Mainly produced in Henan, Shandong, Yunnan, Guangxi and other places, it is one of the main sources of Shanyinhua medicinal materials. Honeysuckle is a commonly used clinical traditional Chinese medicine. It is sweet in taste and cold in nature. It is used to treat carbuncle, sore throat, erysipelas, bloody dysentery, wind-heat cold, and fever. It is an important medicine for clearing away heat and detoxifying. It has inhibitory effect on a variety of pathogenic bacteria and is a broad-spectrum antibacterial traditional Chinese medicine, known as "penicillin in traditional ...

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Problems solved by technology

[0004] The purpose of the embodiments of the present invention is to provide an ultrasonic extraction method of chlorogenic acid from honeysuckle leaves, aiming at solving the problem that honeysuckle has been used as an industrial raw material for extracting chlorogenic acid for a long time, but the price is expensive, and the development and utilization of other plants of the same genus are not enough, especially It is due to insufficient attention and research on the leaves of Lonicera red glandularis. There is a certain amount of chlorogenic acid in each part of Lonicera red glandularis, but the content is different, the order is flower>leaf>stem>root, and the chlorogenic acid in the leaf The content is relatively high, which is 69.63% of the flowers, which is much more than the rhizomes, while the stems and leaves of honeysuckle are far more abundant than the red glandular honeysuckle. It is easy to collect and cheap, so it should be fully utilized.

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