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Method for extracting miraculin from miracle fruit

A kind of miracle fruit and mysterious technology, which is applied in the field of preparation of miracle fruit juice drinks, can solve the problems of low yield and the like, and achieve the effects of product safety, sweet taste, and inhibiting the deepening of color.

Inactive Publication Date: 2013-05-08
万福群
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Narendra established a one-step extraction method with Immobilized Metal-Affinity Chromatography nickel column based on the structural characteristics of polyhistidine residues (easy to bind with nickel) in mysticin. The purity of mysticin obtained by this chromatographic separation method is greater than 95%, but the yield of this method is low, limited to experimental analysis, separation and purification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Remove the leaf stalks from the freshly picked miracle fruit, wash them with sterile water and drain the water within 4 hours, and store them in the refrigerator at -18°C; put the miracle fruit out of the refrigerator into a blender, and then add the fruit quality 3 times of 1-4 ℃ sterile water, stir until the pulp and core are separated, and the whole process is completed within 10 minutes. The stirred mixture was centrifuged at 8000 × g for 20 min at 4 °C. The supernatant is juice serum. Rinse the sediment with 5 times of sterile water at 1-4 °C, and filter with suction to obtain the filter residue as pulp slurry.

[0025] Dissolve sucrose, glucose and vitamins in the fruit juice clear liquid, use 10% citric acid solution to adjust the pH value of the fruit juice clear night to 3.3, mix and stir evenly, and obtain the miracle fruit juice drink; wherein the content of total vitamins in the miracle fruit juice drink is 25 mg / 100ml, the consumption of sucrose is 4.0% o...

Embodiment 2

[0031] Remove the leaf stalks from the freshly picked miracle fruit, wash them with sterile water and drain the water within 4 hours, and store them in the refrigerator at -25°C; put the miracle fruit out of the refrigerator into a blender, and then add the fruit quality 5 times of 1-4 ℃ sterile water, stirred until the pulp and core were separated by 10000, and the whole process was completed within 10 minutes. The stirred mixture was centrifuged at 10000 × g for 26 min at 4 °C. The supernatant is juice serum. Rinse the sediment with 8 times sterile water at 1-4 °C, and filter with suction to obtain the filter residue as pulp slurry.

[0032] Dissolve sucrose, glucose and vitamins in the fruit juice clear liquid, use 10% citric acid solution to adjust the pH value of the fruit juice clear night to 3.3, mix and stir evenly, and obtain the miracle fruit juice drink; wherein the content of total vitamins in the miracle fruit juice drink is 40 mg / 100ml, the consumption of sucro...

Embodiment 3

[0038] Remove the leaf stems from the freshly picked miracle fruit, wash them with sterile water within 4 hours, drain the water, and store them in the refrigerator at -36°C; put the miracle fruit out of the refrigerator into a blender, and then add the fruit quality 6 times of 1-4 ℃ sterile water, stir until the pulp and core are separated, and the whole process is completed within 10 minutes. The stirred mixture was centrifuged at 12000 × g for 30 min at 4 °C. The supernatant is juice serum. Rinse the sediment with 10 times sterile water at 1-4 °C, and filter with suction to obtain the filter residue as pulp slurry.

[0039] Dissolve sucrose, glucose and vitamins in the fruit juice clear liquid, use 10% citric acid solution to adjust the pH value of the fruit juice clear night to 3.4, mix and stir evenly, and obtain the miracle fruit juice drink; wherein the content of total vitamins in the miracle fruit juice drink is 50 mg / 100ml, the consumption of sucrose is 7.0% of the...

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Abstract

The invention discloses a method for extracting miraculin from a miracle fruit. The method comprises the following steps of: (1) preprocessing the miracle fruit; (2) preparing pulp and juice of the miracle fruit; (3) preparing juice beverage of the miracle fruit by adding a sweetening agent and vitamins; (4) selecting a saliferous acidic buffer as an extracting solution, homogeneously blending, and efficiently extracting the miraculin with the help of a high-voltage pulse electric field; and (5) desalting and concentrating a coarse extracting solution of the miraculin by using an ultra-filter membrane with a membrane flux of 10,000Da, and freezing and drying to obtain a miraculin product. The method provided by the invention has the advantages of simple requirements on production equipment and high security; the prepared miraculin has the characteristics of high miraculin purity and strong activity, and is suitable for large-scale industrial production; and the nutrient and delicious fruit juice beverage can be made of the juice of the miracle fruit produced in a preparation process, so that the application range of the miracle fruit is expanded.

Description

technical field [0001] The invention relates to the efficient extraction of miraculin from miraculous fruit and the preparation of miraculous fruit juice drink, in particular to a method for extracting miraculin from miraculous fruit. Background technique [0002] Miracle fruit (Richadella dulcifica) belongs to the genus Miracle Fruit of Sapotaceae. It is an evergreen shrub plant. It is native to the tropical region of West Africa and is naturally distributed in the Congo area of ​​West Africa. my country introduced plantation in the 1960s, and it grows well in tropical and subtropical regions such as Hainan, Yunnan, and Guangxi. The mystery of miracle fruit is that it can turn acidic food into sweet taste within a few hours. Research has proved that the taste-changing component is glycoprotein, called Miraculin. Therefore, its mysterious element can be made into a food aid for acidic foods, and can also be made into a taste-changing agent that meets the sweetness needs of ...

Claims

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Application Information

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IPC IPC(8): A23L1/222A23L2/04A23L2/52A23L27/12
Inventor 赵谋明万福群
Owner 万福群
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