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HOST2 kit for assisting in early diagnosis of epithelial ovarian cancer and application of HOST2 kit

A technology for early diagnosis of ovarian cancer, applied in the field of medical biological detection, can solve the problem of no HOST2EOC biological markers, and achieve the effect of preventing disease progression, strong operability, and less trauma

Inactive Publication Date: 2014-04-02
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Currently, no other assays for HOST2 are available, and there is no literature on HOST2 as a biomarker for EOC

Method used

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  • HOST2 kit for assisting in early diagnosis of epithelial ovarian cancer and application of HOST2 kit
  • HOST2 kit for assisting in early diagnosis of epithelial ovarian cancer and application of HOST2 kit
  • HOST2 kit for assisting in early diagnosis of epithelial ovarian cancer and application of HOST2 kit

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: preparation kit of the present invention

[0034] The DNA sequence of HOST2 is shown in SEQ ID NO:1.

[0035] The upstream and downstream primers of its specific Real-time PCR were designed as follows by Primer5 software, and the primers were synthesized by Invitrogen Company of the United States, with a purity of PAGE grade. 2 O was dissolved to a final concentration of 10 μM.

[0036] Upstream primer 5`-GGACAGGTCCCTTGTTTCAA-3` (as shown in SEQ ID NO: 2)

[0037] Downstream primer 5'-CTGGTCTTTTCCTTGCCTCTG-3' (as shown in SEQ ID NO: 3)

[0038] Prepare a kit including the following components: total RNA extraction reagent, chloroform, absolute ethanol, total RNA reverse transcription primer (100 μl / tube concentration: 10 μM), specific HOST2 upstream primer (100 μl / tube concentration: 10 μM), Downstream primer (100μl / tube concentration: 10μM), DEPC H 2 O (1000μl / tube), ddH 2 O (2000μl / tube)

[0039] DEPC H 2 Configuration of O: Use purchased DEPC (di...

Embodiment 2

[0041] Embodiment 2: the detection of kit of the present invention

[0042] 1. Collection and processing of serum or plasma samples:

[0043] Since serum or plasma has the advantages of convenient sampling and continuous detection, detection of biomarkers in patients suspected of EOC before surgery from serum or plasma can improve the early diagnosis of EOC to a new level.

[0044] Serum or plasma samples were collected in the inpatient department of Shanghai Changhai Hospital, and all patients had no blood, kidney, tumor and other diseases that may affect serum or plasma levels. Sample collection: Collect about 3~6ml of peripheral blood from the patient and put it into an anticoagulant tube containing 360μl EDTA, mix it upside down, let it stand for about half an hour, and centrifuge to get the supernatant to get plasma; collect about 3~6ml of peripheral blood from the patient and release it. Put it into a vacuum coagulation tube, invert it several times to fully combine, le...

Embodiment 3

[0065] Example 3: Expression of HOST2 in peripheral blood plasma of normal people and EOC patients

[0066] Firstly, the preoperative serum and plasma levels of HOST2 in EOC patients were detected respectively, and normal subjects were used as controls, a total of 20 cases. The results of Real-time PCR found that there was no statistically significant difference between the plasma and serum data. The content of HOST2 was extremely weak, and some samples could not even be detected, but the content of HOST2 in the experimental group was much higher than that in the control group by about 13 times (see figure 1 and figure 2 ).

[0067] Then, a total of 43 patients who were initially suspected of EOC based on age, medical history and local signs were selected, and serum samples were collected from these patients before and after surgery. Among them, 22 patients were pathologically diagnosed as EOC during and after the operation. The serum HOST2 levels of the 22 patients were de...

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Abstract

The invention relates to the technical field of medical biological detection and provides an HOST2 detection kit for assisting in early diagnosis of an epithelial ovarian cancer (EOC). The HOST2 detection kit consists of a reverse transcription system, a primer system and an amplification system, wherein HOST2 specific qRT-PCR (quantitative real-time polymerase chain reaction) upstream primer is shown as SEQ ID No: 2; and a downstream primer is shown as SEQ ID NO: 3. The kit adopts a qRT-PCR method, detects an HOST2 content of plasma or serum for assistance of the early diagnosis of the EOC, and has the characteristics of small trauma, high operability and the like. The invention further provides a detection method of the HOST2 detection kit for assisting in the early diagnosis of the EOC.

Description

technical field [0001] The invention relates to the technical field of medical biological detection, in particular to a HOST2 kit for assisting the early diagnosis of epithelial ovarian cancer, and its application and detection method in assisting the early diagnosis of epithelial ovarian cancer. Background technique [0002] Epithelial ovarian cancer (Epithelial Ovarian Cancer, EOC) is the most common ovarian cancer, accounting for about 80-90%, is currently the highest mortality rate of gynecological malignancies, up to 70%. Because the ovary is located in the deep part of the pelvic floor, it causes many difficulties in the early diagnosis of ovarian cancer. At the time of clinical diagnosis, 70% of ovarian cancer is already in the advanced stage (Luesley, D., Lawton F., Blackledge G., Hilton C., Kelly K., Rollason T., Wade-Evans T., Jordan J., Fielding J., Latief T.. Failure of second-look laparotomy to influence survival in epithelial ovarian cancer. Lancet. 1988, 2(861...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 刘善荣惠宁高原刘淑鹏余喜亚胡晶晶
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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