Method for detecting mineral nutrient cycling from cotton seedling stage to cotton flowering and boll-forming stage by applying pressure

Abstract

The invention provides a method for detecting mineral nutrient cycling from a cotton seedling stage to a cotton flowering and boll-forming stage by applying pressure. The method is characterized in that (1) transpiration of the cotton plants can be carried out normally; (2) transportation of photosynthetic products and mineral nutrients of cotton phloem are not broken, so that the nutrient cycling process in the cotton body can be carried out still; (3) under a condition that no relatively good method for studying the phloem transportation, the method can directly measure nutrient content in the cotton xylem sap for mathematical calculation, so that the cycling process of the nutrients in the plant body at a certain time can be known quantitatively; (4) plants for collecting the xylem sap can be used repeatedly, so that a change condition of the xylem sap in different growth stages of the same plant can be tracked and known, and influence on composition of the xylem sap by studying nutrient supply change. Besides, the method can be used for quantitatively studying distribution and cycling process in plant bodies of elements such as N, Na, Mg, P and the like in different plants.

Description

technical field [0001] The invention relates to a testing technology of plant mineral nutrients, which applies certain pressure to the root system of a normally growing cotton plant to study the transportation and circulation process of the absorbed nutrients in the body, which has important scientific and practical value. Background technique [0002] The absorption and circulation of mineral nutrients is the link of plant growth. The absorption of mineral nutrients in the soil is through the plant root-xylem-shooting part-phloem, which is usually called the cycle of mineral nutrients. It is through the circulation and recycling of mineral nutrients that plants provide the nutrients needed by plants for plant growth and development through root absorption, provide mass flow driving force for xylem and phloem, and provide light and nutrients for roots. This method is to study the circulation of mineral nutrients in cotton under greenhouse conditions. Through this research, t...

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Problems solved by technology

This method collects a large amount of juice, but the pressure should not be too high, otherwise the xylem juice will be diluted, and the nature of water flow and nutrient supply in the xylem of different plants is different, so it is difficult to know exactly how much pressure to apply

The common disadvantage of the above methods is to complete the collection of samples on the premise of destroying the plants, which destroys the normal metabolism of the plants and cannot accurately reflect the physiological activities of the complete plants.

This is limited to the study of the mineral nutrient absorption and circulation process under the natural conditions of plants. At the same time, it takes a long time to collect the juice and cannot be collected directly, which will cause large errors in experimental data.

[0006] The device used and the method of collecting xylem juice have also been successfully applied to tobacco and corn. The relevant literature has been introduced in the article "Methods for Studying Mineral Nutrient Cycle in Plants" issued by Lu Yongxian, but it is not applicable to cotton. There is no research report on the application technology, mainly because the leaves and bells of cotton fall off seriously after the boll opening period, and the leaves begin to decline, so this method is not suitable for research

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