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Production and fermentation process for citric acid

A fermentation process and citric acid technology, applied in the direction of fermentation, can solve the problems of difficulty in ensuring the first-in first-out property of the feed liquid, reducing the utilization rate of nutrients, and troublesome fermentation tanks, so as to improve the conversion rate of sugar and acid and the utilization rate of equipment, Avoid loss and contamination, avoid the effect of low total sugar concentration

Active Publication Date: 2013-04-24
RIZHAO JINHE BOYUAN BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Continuous fermentation refers to the fermentation process in which fresh medium is added to the fermenter at a certain speed, and the culture liquid is flowed out at the same speed, so that the liquid volume in the fermenter is kept constant. Since citric acid fermentation is aerobic fermentation, so In the continuous culture process, it is difficult to ensure the first-in-first-out nature of the feed liquid, thereby reducing the utilization rate of nutrients; at the same time, it causes the loss of some Aspergillus niger strains in the period of vigorous acid production, and continuous fermentation is easy to be infected with bacteria. A fermenter is very troublesome
Batch fermentation refers to a closed system in which a limited amount of nutrients are put in, and then a small amount of microbial strains are inserted for cultivation to allow the microorganisms to grow and reproduce. Under certain conditions, only one growth cycle of fermentation is completed. As the fermentation proceeds, the total sugar concentration in the fermentation system gradually decreases, resulting in low fermentation coefficient, long cycle, high energy consumption, high cost, and low utilization rate of equipment, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A kind of citric acid production fermentation process, its concrete steps are:

[0020] (1) Put the corn liquefaction solution into the seed tank, add ammonium sulfate accounting for 0.025% of the medium volume as an inorganic nitrogen source, heat up to 121°C for 30 minutes, cool down to 37°C, insert spores for seed liquid cultivation, Seed liquid seed age is 28h;

[0021] (2) Put the filtered corn liquefaction liquid, corn liquefaction liquid, cottonseed flour slurry and soybean meal flour slurry into the fermenter at a volume ratio of 16:2:1:1, start stirring to mix the medium evenly, and heat up to 90°C Sterilize for 30 minutes, the initial total sugar concentration is 21.50g / 100mL, then cool down to 37°C, and add acid protease at 10 enzyme units per gram dry basis;

[0022] (3) Mix the filtered corn liquefaction clear liquid and corn liquefaction liquid at a volume ratio of 6-10:1, saccharify, add molasses to it to adjust the total sugar concentration to 37g / 100mL...

Embodiment 2

[0026] A kind of citric acid production fermentation process, its concrete steps are:

[0027] (1) Put the corn liquefaction solution into the seed tank, add ammonium sulfate accounting for 0.025% of the medium volume as an inorganic nitrogen source, heat up to 121°C for 30 minutes, cool down to 37°C, insert spores for seed liquid cultivation, Seed liquid seed age is 29h;

[0028] (2) Put the filtered corn liquefaction liquid, corn liquefaction liquid, cottonseed flour slurry and soybean meal flour slurry into the fermenter at a volume ratio of 16:2:1:1, start stirring to mix the medium evenly, and heat up to 90°C Sterilize for 30 minutes, the initial total sugar concentration is 20.50g / 100mL, then cool down to 37°C, and add acid protease at 10 enzyme units per gram dry basis;

[0029] (3) Mix the filtered corn liquefaction clear liquid and corn liquefaction liquid at a volume ratio of 6-10:1, saccharify, add molasses to it to adjust the total sugar concentration to 35g / 100mL...

Embodiment 3

[0033] A kind of citric acid production fermentation process, its concrete steps are:

[0034] (1) Put the corn liquefaction solution into the seed tank, add ammonium sulfate accounting for 0.025% of the medium volume as an inorganic nitrogen source, heat up to 121°C for 30 minutes, cool down to 37°C, insert spores for seed liquid cultivation, Seed liquid seed age is 30h;

[0035] (2) Put the filtered corn liquefaction liquid, corn liquefaction liquid, cottonseed flour slurry and soybean meal flour slurry into the fermenter at a volume ratio of 16:2:1:1, start stirring to mix the medium evenly, and heat up to 90°C Sterilize for 30 minutes, the initial total sugar concentration is 20.00g / 100mL, then cool down to 37°C, and add acid protease at 10 enzyme units per gram dry basis;

[0036] (3) Mix the filtered corn liquefaction clear liquid and the corn liquefaction liquid at a volume ratio of 6-10:1, saccharify, add molasses to it to adjust the total sugar concentration to 40g / 1...

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PUM

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Abstract

The invention provides a production and fermentation process for citric acid. The process is characterized in that feeding liquid is refilled into a fermentation tank during the fermentation process of citric acid. According to the invention, through fed batch of the feeding liquid with high concentration of sugar during fed batch fermentation, the concentration of the acid through fermentative production is improved, and inhibition of high initial sugar concentration on fermentation of the citric acid is avoided. With the production and fermentation process provided by the invention, the concentration of the acid through fermentative production is improved, fermentative production intensity is enhanced, the conversion ratio of sugar to the acid during fermentation and the utilization rate of equipment are increased, and thus, economic benefits of an enterprise are increased and competitiveness of the enterprise is improved.

Description

technical field [0001] The invention provides a fermentation process for producing citric acid. Background technique [0002] Citric acid, scientific name 2-hydroxypropane-1,2,3-tricarboxylic acid, molecular formula C 6 h 8 o 7 (anhydrous), is an organic acid widely used in food, medicine and chemical industry. With the development of the economy, the demand for citric acid in all walks of life will rise steadily. Of course, the challenges and opportunities faced by various enterprises are also increasing. [0003] At present, citric acid production mainly includes continuous fermentation and batch fermentation. Continuous fermentation refers to the fermentation process in which fresh medium is added to the fermenter at a certain speed, and the culture liquid is flowed out at the same speed, so that the liquid volume in the fermenter is kept constant. Since citric acid fermentation is aerobic fermentation, so In the continuous culture process, it is difficult to ensure ...

Claims

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Application Information

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IPC IPC(8): C12P7/48
Inventor 寇光智李昌涛王有旭周一江周昊秦绪斌
Owner RIZHAO JINHE BOYUAN BIOCHEM
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