Genetically engineered bacteria for producing N-acetylneuraminic acid as well as construction method and application thereof

Abstract

The invention discloses genetically engineered bacteria for producing N-acetylneuraminic acid as well as a construction method and application thereof. A method for preparing the genetically engineered bacteria for producing N-acetylneuraminic acid comprises the following step of: introducing genes associated with the N-acetylneuraminic acid synthesis into host bacteria so as to obtain recombinant bacteria for producing N-acetylneuraminic acid, wherein the genes associated with the N-acetylneuraminic acid synthesis are coding genes of N-acetylglucosamine and 2-isomerase and a coding gene of N-nacetylneuraminic acid aldolase. The genetically engineered bacteria for producing N-acetylneuraminic acid constructed by the invention have the following advantages that the used raw materials are relatively cheap and liable to realize industrial mass product, so that the genetically engineered bacteria play an important role in the production of N-acetylneuraminic acid in the future.

Description

technical field [0001] The invention relates to a genetically engineered bacterium producing N-acetylneuraminic acid and its construction method and application. Background technique [0002] N-acetylneuraminic acid (N-acetyl-D-neuraminic acid, Neu5Ac) is the most representative member of the sialic acid family, a pyranose composed of 9 carbon atoms, has a wide range of biological functions, in the treatment of Influenza, neurological diseases, inflammation and tumors have important medical value, and have broad application prospects and high market value, especially in the prevention and treatment of influenza (including avian influenza H5N1 and 2009 H1N1). good effect. [0003] At present, the sources of N-acetylneuraminic acid are limited, and there are few studies on industrial production. The traditional production methods lead to its high price, which greatly limits the related research and development and application. N-acetylneuraminic acid can be extracted from na...

AI Technical Summary

Problems solved by technology

This method does not need to separate and purify the enzyme. At the same time, due to the protection of the cell membrane, the enzyme is relatively more stable and the cofactor is easy to regenerate.

However, since two kinds of bacteria expressing different enzymes need to be cultivated separately, and the bacteria are concentrated and then mixed, this mode is difficult to industrialize. At the same time, the substrate and product enter and exit the cell many times and are blocked by the cell wall, thus affecting the catalytic reaction efficiency and transformation. Rate

[0004] It can be seen from the above several methods for synthesizing N-acetylneuraminic acid that the existing production methods have low output, high cost, and difficulty in scale expansion, resulting in low productivity of Neu5Ac, which is difficult to meet future market demand.

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