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Corticogenesis of human pluripotent cells

A technology of human pluripotent stem cells and cell cortex, applied in the field of cortical generation of human pluripotent cells, can solve the problem of not showing the effect of sonic hedgehog

Inactive Publication Date: 2013-04-17
CAMBRIDGE ENTERPRISE LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Although some types of cortical neurons have been previously shown to be generated in aggregate cultures of human ES cells (US20100166720A1; Eiraku, M., et al. Cell stem cell 3, 519-532 (2008); Li, X.J., et al. al.Development136,4055-4063(2009)), but did not show the effect of sonic hedgehog inhibition on corticogenesis of human pluripotent stem cells in monolayer culture

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  • Corticogenesis of human pluripotent cells
  • Corticogenesis of human pluripotent cells
  • Corticogenesis of human pluripotent cells

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Experimental program
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Embodiment Construction

[0205] experiment

[0206] method

[0207] Human pluripotent stem cell culture

[0208] Human ES cell research was approved by the Steering Committee of the UK Stem Cell Bank (UK Stem Cell Bank) and the Use of Stem Cell Lines (UK Code of Practice for the Use of Human Stem Cell Lines). Cell Lines) to carry out. hES (H9, WiCell Research Institute, and Edi2, a gift from Jenny Nichols, Cambridge Center for Stem Cell Research) and hiPS cell lines (DS1-iPS4, Harvard Stem Cell Institute22) were cultured according to standard methods13 in the presence of mitomycin Treatment was performed on mouse embryonic fibroblasts (MEFs). Briefly, cells were maintained in hESC medium (all components were from Invitrogen unless otherwise noted): DMEM / F12 with 20% KSR, 6 ng / ml of FGF2 (PeproTech), 1 mM of L-Gln, 100 μM of non-essential amino acids, 100 μM of 2-mercaptoethanol, 50 U / ml of penicillin, and 50 mg / ml of streptomycin.

[0209] directed differentiation into the cerebral cortex...

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Abstract

This invention relates to in vitro methods for the induction of corticogenesis in human pluripotent cells, such as iPS cells, by culturing the cells under conditions which stimulate retinoid signalling and inhibit TGFss superfamily signalling. This may be useful in production of cortical neurons, in particular patient- specific cortical neurons; the modelling of juvenile and adult-onset neurological diseases; and the development of therapeutics.

Description

technical field [0001] The present invention relates to the in vitro induction of corticogenesis in pluripotent human cells. Background technique [0002] The cerebral cortex (cortex, cortex) is the synthesis and executive center of the mammalian central nervous system and constitutes more than three-quarters of the human brain (Mountcastle, V.B. The Cerebral Cortex, (Harvard University Press, Cambridge, Mass. 1998). Brain Diseases of the cortex are a major cause of morbidity and mortality in children and adults, ranging from developmental disorders such as epilepsy and autism to late-life neurodegenerative disorders such as Alzheimer's disease. Rodent models of cerebral cortex development, Function, and basic features of disease have been extensively studied. However, the primate, especially the human cerebral cortex differs from that of rodents in many respects (Finlay, B.L. et al. Science 268, 1578-84 (1995)). Except In addition to the marked increase in the size of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/079C12N5/0797C12Q1/02A61K35/12A61K35/30A61K35/545
CPCC12N5/0619C12N2500/44C12N2501/15C12N2501/33C12N2501/385C12N2501/999C12N2503/02C12N2506/02C12N2506/45C12N2533/32C12N2533/52G01N33/5058G01N33/6896G01N2800/2821C12N5/0623A61P25/00A61P25/28A61P43/00
Inventor 弗雷德里克·约翰·利夫西施熠辰
Owner CAMBRIDGE ENTERPRISE LTD
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