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Extracting process for efficiently extracting polysaccharide of enteromorpha

The technology of prolifera polysaccharide and extraction process is applied in the field of extraction process for efficient extraction of prolifera polysaccharide, can solve the problems of short extraction time, low extraction efficiency, inability to meet efficiency requirements, etc., and achieves shortening extraction time, efficient extraction, overcoming Effects of Organic Residue Problems

Active Publication Date: 2013-04-17
FUJIAN HAIXING HEALTH FOOD
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As can be seen from Table 1, the extraction time of hot water extraction-ultrasonic method and hot water extraction-microwave assisted method is short, but the polysaccharide yield is the lowest, and the polysaccharide yield of simple hot water extraction method is also low. Therefore, the hot water extraction method, hot water extraction-ultrasonic method and hot water extraction-microwave-assisted method cannot meet the efficiency requirements in production
[0009] Compared with the first three extraction methods, the cellulase extraction method and hot water extraction-papain combined extraction method have greatly improved the polysaccharide yield, but compared with the increase in extraction time, the extraction efficiency is very low. low, and the extraction process of the above five Enteromorpha polysaccharides does not involve desalination or deproteinization steps, and the purity of the obtained polysaccharides is low

Method used

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  • Extracting process for efficiently extracting polysaccharide of enteromorpha
  • Extracting process for efficiently extracting polysaccharide of enteromorpha

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Embodiment 13~24

[0029] Embodiments 13-24: Correspondingly, on the basis of the extraction process I in the above-mentioned embodiments 1-12, carry out the embodiments 13-24 according to the process II.

Embodiment 25~27

[0030] Examples 25-27: On the basis of Example 24, a dialysis membrane of 6000-7000 Da was used in the desalination step, and the dialysis was performed for 5-7 hours; 0.02g / ml Enzymolysis at 25~45℃ for 0.5~1h. Now the difference of embodiment 25~27 is specifically listed as follows:

[0031] The concrete reaction condition of table 3 embodiment 25~27

[0032] Example Dialysis membrane pore size (Da) Protease concentration (g / ml) Enzyme pH Enzymolysis temperature (°C) 25 6000 0.02 4.5 45 26 6500 0.017 5.3 37 27 7000 0.001 6 25

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Abstract

The invention provides an extracting process for efficiently extracting polysaccharide of enteromorpha. In a process of adding water to extract, the polysaccharide of enteromorpha is extracted by at least two of a microwave method, an ultrasonic method and an enzyme method, wherein in the ultrasonic method, the ultrasonic frequency is 3800 MHz-7500MHz, the extracting time is one minute to three minutes, and the pH value is 5.0-5.5; in the microwave method, the microwave frequency is 55kHz-75kHz, the extracting time is one minute to three minutes and the pH value is 5.0-5.5; in the enzyme method, the adding amount of the enzyme is 0.8%-2%, the pH value is 5.0-6.0, the treatment time is 1.5hours-2hours and the reaction temperature is 25 DEG C to 37 DEG C; and in a solid-liquid separating step, the slag of the extracted liquid is removed by adopting a screen cloth of 150-250 meshes. According to the extracting process disclosed by the invention, the polysaccharide of enteromorpha can be extracted within a short time.

Description

technical field [0001] The invention relates to the field of seaweed extraction, in particular to an extraction process for efficiently extracting enteromorpha polysaccharides. Background technique [0002] Enteromorpha, also known as moss, belongs to Ulvaceae (including Ulva), and is a large-scale wild green algae in the ocean. Seaweed is an important part of my country's rich plant resources. With the development and utilization of seaweed resources, related seaweed The research on pharmacological functions and biologically active ingredients continues to expand and deepen. [0003] Studies at home and abroad have shown that Enteromorpha polysaccharides have a wide range of biological activities and medicinal functions, and have good blood lipid-lowering, anti-tumor, anti-virus, anti-bacterial, immune-regulating, anti-oxidation, anti-fatigue, hypoglycemic and anti-radiation properties. a biological activity. [0004] At present, there are few studies on Enteromorpha polys...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 陈灿坤
Owner FUJIAN HAIXING HEALTH FOOD
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