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Salt-resistant gene SbSAP14 of sorghum bicolor and application thereof

A technology of sbsap14 and sorghum, which is applied in the application field of SbSAP14 gene in improving the salt resistance of plants, and can solve the problems of increased sensitivity to salt and drought stress, etc.

Inactive Publication Date: 2013-04-17
SOUTH CHINA NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Transgenic tobacco plants overexpressing ZFP177 (OsiSAP9) have enhanced tolerance to low temperature and high temperature stress, but also enhanced sensitivity to salt and drought stress (Huang et al. 2008)

Method used

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  • Salt-resistant gene SbSAP14 of sorghum bicolor and application thereof
  • Salt-resistant gene SbSAP14 of sorghum bicolor and application thereof
  • Salt-resistant gene SbSAP14 of sorghum bicolor and application thereof

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Embodiment 1

[0030] 1. Sorghum SbSAP14 gene cloning

[0031] 1. Primer design

[0032] Rice published in GenBank database OsiSAP14The gene sequence was used as a query probe, and the homologous sequence alignment method was used to obtain the corresponding OsiSAP14 Candidate genes with high similarity are named SbSAP14 , its nucleotide sequence is shown in SEQ ID NO:1, and the encoded protein is shown in SEQ ID NO:2. according to SbSAP14 Gene sequence characteristics, using Primer Premier 5.00 to assist in the design of specific primers (Table 1).

[0033]

[0034] 2. Sorghum SbSAP14 PCR amplification of genes

[0035] The total RNA of sorghum root was extracted using Trizol reagent, and the method was referred to the instructions provided by Invitrogen Biological Company. cDNA was obtained by reverse transcription according to the instruction of Invitrogen reverse transcriptase Super Script TM III RT. The obtained cDNA was used as a template, and specific primers SbSAP14 F...

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Abstract

The invention discloses a salt-resistant gene SbSAP14 of sorghum bicolor and an application thereof. The salt-resistant gene SbSAP14 of the sorghum bicolor has a nucleotide sequence as shown in SEQIDNO: 1; and the coded protein has an amino acid sequence as shown in SEQIDNO: 2. According to the invention, the salt-resistant gene SbSAP14 of the sorghum bicolor can improve the salt resistance of plants, so that a theoretical basis can be provided for effects of the SbSAP14 gene in the salt stress resisting process of the sorghum bicolor, a theoretical basis can be provided for establishing a stress signal transduction pathway network of the sorghum bicolor, and a novel gene resource is provided for utilizing the SbSAP14 gene to improve the sorghum bicolor or other important grain crops and economic crops in the future so as to obtain a novel variety of salt-resistant plant.

Description

technical field [0001] The invention belongs to the field of genetic engineering, in particular to sorghum salt resistance genes SbSAP14 ,contain SbSAP14 Gene recombinant expression vector, SbSAP14 the protein encoded by the gene, and SbSAP14 Application of genes in improving plant salt tolerance. Background technique [0002] Abiotic stresses, such as drought, salt, extreme temperature, high light, heavy metals, etc., affect the growth and yield of crops, pose a huge threat to agricultural production, and seriously affect the sustainable development of agriculture. Abiotic stress is the main cause of crop yield reduction worldwide, with an average yield reduction of over 50% for the most important crops (Vij and Tyagi 2007). Breeding stress-resistant plant varieties has become increasingly important as crop cultivation expands into areas that are not suitable for crop growth (Kathuria et al. 2007). Transgenic approaches offer new ways to increase plant tolerance to abi...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/21C12N15/84A01H5/00
Inventor 王小菁王瑛华孙姝兰彭建宗
Owner SOUTH CHINA NORMAL UNIVERSITY
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