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Preparation and quality control method of gangsong general flavones

A quality control method and a technology of total flavonoids, applied to medical preparations containing active ingredients, measuring devices, pharmaceutical formulas, etc., can solve the problem of wasting medicinal resources, pine pine is not used much, and pine pine is not fully developed Use and other problems to achieve good application prospects, fast separation speed, and improve market competitiveness.

Active Publication Date: 2013-04-17
GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Pinus pinus plants are widely distributed in my country, and the resources are quite rich, but there are not many applications of pinus pinus pinus at present, and most of the applications are limited to the extraction of volatile oil. application, Gangsong medicinal materials have not been fully developed and utilized, seriously wasting medicinal material resources

Method used

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  • Preparation and quality control method of gangsong general flavones
  • Preparation and quality control method of gangsong general flavones
  • Preparation and quality control method of gangsong general flavones

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039]Take 1000g of dry stems and leaves of Pinus pine, add 10L of 30% ethanol, heat and reflux for extraction 3 times, each time for 1 hour, combine the extracts, filter, and recover the filtrate under reduced pressure until it has no ethanol smell, add water to dissolve to 3000mL, shake well to obtain Concentration is 0.33g of crude drug amount / mL of Gangsong pine extract. Take 150mL of the treated AB-8 macroporous adsorption resin, wet-pack the column, absorb 300mL Pinus pine extract at a sample flow rate of 0.5BV / h, and elute with 2BV of water until the eluent is basically clear. Discard the water eluate, elute with 5BV 50% ethanol at a flow rate of 2BV / h, collect 5BV eluate, recover ethanol under reduced pressure, concentrate to dryness, and vacuum dry at 60°C to obtain the total flavonoids extract of Pinus pine, and determine , Calculated, the total flavonoid content is 52.3%.

Embodiment 2

[0041] Take 1000g of dry stems and leaves of Pinus pine, add 15L of 50% ethanol, heat and reflux for extraction 3 times, 2 hours each time, combine the extracts, filter, and recover the filtrate under reduced pressure until it has no ethanol smell, add water to dissolve to 2000mL, shake well, and get The concentration is 0.5g crude drug amount / mL Gangsong pine extract. Take 100mL of D101 macroporous adsorption resin that has been treated, and wet-pack the column. Adsorb 400mL of Pinus pine extract at a sample flow rate of 1BV / h, and elute with 2BV of water until the eluent is basically clear, and then eluted with water. The liquid was discarded, eluted with 6BV 70% ethanol at a flow rate of 3BV / h, collected 6BV eluate, recovered ethanol under reduced pressure, concentrated to dryness, and dried in vacuum at 60°C to obtain the total flavonoids extract of Pinus pine, determined, calculated, The total flavonoid content is 51.1%.

Embodiment 3

[0043] Take 1000g of dry stems and leaves of Pinus pine, add 15L of 70% ethanol, heat and reflux for extraction 3 times, each time for 3 hours, combine the extracts, filter, and recover the filtrate under reduced pressure until it has no ethanol smell, add water to dissolve to 1250mL, shake well to obtain The concentration is 0.8 g of crude drug / mL extract of Pinus pine. Take 100mL of the treated AB-8 macroporous adsorption resin, and wet-pack the column. Adsorb 500mL of Pinus pine extract at a sample flow rate of 2BV / h, and elute with 2BV of water until the eluent is basically clear. Discard the eluate, elute with 10BV of 50% ethanol at a flow rate of 5BV / h, collect 8BV of eluate, recover ethanol under reduced pressure, concentrate to dryness, and dry under vacuum at 60°C to obtain the total flavonoids extract of Pinus pine, determined, Calculated, the total flavonoid content is 54.0%.

[0044] Quality control method of total flavonoids of Gangpine:

[0045] TLC identificat...

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Abstract

The invention discloses a general flavone extract obtained by extracting, concentrating, separating and drying dried plant gangsong stems and leaves. According to the invention, general flavones are qualitatively discriminated by a thin-layer chromatography, and the content of gangsong general flavones is detected through an ultraviolet-visible spectrophotometry. The general flavone extract is accurate and stable in method, convenient to operate, and can be suitable for requirements of the industrial production, and can effectively control the quality of the gangsong general flavones. The gangsong general flavone extract provided by the invention is low in toxicity, and has the pharmacological effects of anti-inflammation, bacteriostasis, analgesia and the like; and furthermore, the gangsong general flavone extract is reasonable and stable in preparation method, short in production period, and suitable for industrial production. The gangsong general flavone extract has a good development foreground.

Description

technical field [0001] The invention relates to the field of preparation of flavonoid extracts, in particular to a preparation and quality control method of total flavonoids of pine pine. Background technique [0002] Natural plant extracts are formed by using plants as raw materials, according to the purpose of extraction, through the process of physical and chemical extraction and separation, and directional acquisition and concentration of one or more active ingredients in plants without changing the structure of the active ingredients. product. Compared with traditional herbal medicines and Chinese patent medicines, Chinese medicine extracts at least know one or one type of active ingredients, have clear quantitative indicators, and most of the harmful ingredients are removed, thus greatly improving the safety compared with traditional herbal medicines and Chinese patent medicines. In addition, traditional Chinese medicine extracts also incorporate new modern pharmaceut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/61A61P29/00A61P31/04G01N30/02G01N21/33
Inventor 刘布鸣邱宏聪黄艳林霄孙翠
Owner GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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