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Real-time fluorescence PCR (Polymerase Chain Reaction) detection method for components of apricot in food and beverage

A real-time fluorescence and component detection technology, applied in the direction of fluorescence/phosphorescence, material excitation analysis, biochemical equipment and methods, etc., can solve the problems of ensuring the stability and specificity of the method, improve the detection efficiency and sensitivity, and be applicable performance, anti-pollution effect

Inactive Publication Date: 2014-05-07
郑秋月 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detection method of apricot components in food and beverages in my country is still blank, and there are many difficulties in ensuring the stability and specificity of the method, which is also the bottleneck restricting the establishment of the method

Method used

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  • Real-time fluorescence PCR (Polymerase Chain Reaction) detection method for components of apricot in food and beverage
  • Real-time fluorescence PCR (Polymerase Chain Reaction) detection method for components of apricot in food and beverage
  • Real-time fluorescence PCR (Polymerase Chain Reaction) detection method for components of apricot in food and beverage

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Samples to be tested: Positive control, negative control, and blank control were set up during the testing process. The DNA extracted from the apricot component sample was used as a positive control, the DNA extracted from a sample known not to contain apricot components was used as a negative control, and sterilized water was used as a blank control. Two parallel reaction systems were set up for each group.

[0047] (2) Detection method

[0048] The following DNA extraction methods are all routine operations of those skilled in the art, and all reagents and solutions are prepared by conventional means or obtained from commercial means.

[0049] (1) DNA extraction of liquid samples such as fruit juice

[0050] Invert liquid samples such as fruit juice upside down evenly, fully mix the sediment in the lower layer with the liquid in the upper layer, and then weigh the sample. Take about 30mL of liquid into a clean petri dish, vacuum freeze-dry; weigh 0.2g of freeze-...

Embodiment 2

[0083] Embodiment 2 specificity test

[0084] Use this standard method to test samples (see Table 3 for sample details), Liaoning Provincial Institute of Fruit Tree Science, National Fruit Tree Germplasm Xiongyue Li, Apricot Garden, Dalian Academy of Agricultural Sciences Research Institute, and major supermarkets and markets in Dalian, etc. , the samples mainly include: common apricot, Siberian apricot, Liao apricot, purple apricot, Zhidan apricot, Limei apricot, Tibetan apricot, plum, a total of 8 kinds of apricot; plum, peach, cherry, apple, strawberry, pear, apple, banana, crabapple , orange, blueberry, carambola, chestnut, kiwi, pecan, kumquat, peach, fresh lychee, grape, cantaloupe, hawthorn, citrus, mango, cantaloupe, sand date, pineapple, pomelo, bayberry, etc. DNA samples.

[0085] Partial results of apricot component-specific detection are as follows: image 3 and shown in Table 3.

[0086] Table 3 Apricot component detection specificity test results

[0087] ...

Embodiment 3

[0091] Embodiment 3 detection sensitivity and addition test

[0092]The addition test of primary processed products was done with apple as the substrate. After the apricot and apple fruits were chopped and ground, the apricot pulp was added to the apple pulp and mixed evenly to form a 10% added sample. Add to new apple pulp in this ratio until the addition samples containing 200mg / kg, 100mg / kg, 50mg / kg, 20mg / kg and 10mg / kg of apricot pulp are formed respectively. Mix well during addition to ensure even distribution of the sample in the apple flesh.

[0093] According to the result judgment standard described in embodiment 1, the amplification curve is judged; Add test result: as Figure 4 The sensitivity test results of the addition experiment of primary processed products of apricot ingredients show that apple pulp is used as the matrix of primary processed products, and apricot pulp is added respectively to make the added concentrations of 200mg / kg, 100mg / kg, 50mg / kg, 20mg...

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Abstract

The invention discloses a primer and a probe for detecting components of apricot as well as a use method and an application thereof. Aiming at ribosome 18S rRNA gene sequence of apricot, the method designs the primer and probe for detecting apricot gene; and components of apricot in food and beverage are quantitatively detected by a real time PCR (Polymerase Chain Reaction) method. The real-time fluorescence PCR quantitative detection method for components of apricot in food and beverage is of significance to improve the detection efficiency and agility of the components of apricot in food and beverage, lower the detection cost, detect related bogus products in market, and monitor safety of food and improve the inspection and quarantine technology. The method has a wide development prospect.

Description

technical field [0001] The invention belongs to the field of food quality control, in particular to a real-time fluorescent PCR identification method for apricot components in food and beverages. Background technique [0002] Apricots are generally cultivated in temperate regions of the world. Apricots are native to China and are planted in parts of Central Asia, Southeast Asia, Southern Europe and North Africa. In addition to being eaten fresh, apricot meat can also be processed into preserved apricots, canned apricots, dried apricots, apricot juice, apricot wine, apricot green plum, apricot plum, apricot paeonol, etc.; almonds can be made into almond cream, Almond milk, almond cheese, almond paste, almond snacks, almond pickles, etc. [0003] In recent years, the media has continuously exposed that the prices of processed apricot products (such as almond milk, almond drinks, etc.) sold by some merchants are obviously much lower than those in shopping malls, even lower tha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11G01N21/64
Inventor 郑秋月简中友姜丽曹际娟
Owner 郑秋月
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