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miR-21 compound of target microglial cell and preparation method and application of miR-21 compound

A technology of microglia and mir-21, which is applied in the direction of non-active ingredients of polymer compounds, drug combinations, and pharmaceutical formulations, can solve problems such as nerve cell damage and accelerate central nervous system diseases, and achieve inhibition of activation and occurrence Or the effect of worsening or increasing the intake rate

Inactive Publication Date: 2014-06-11
THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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Problems solved by technology

However, studies have shown that overactivated microglia may accelerate the process of some central nervous system diseases, and the cytotoxic factors released by them will also cause further damage to nerve cells

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  • miR-21 compound of target microglial cell and preparation method and application of miR-21 compound
  • miR-21 compound of target microglial cell and preparation method and application of miR-21 compound
  • miR-21 compound of target microglial cell and preparation method and application of miR-21 compound

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Embodiment Construction

[0016] In order to make the object, technical solution and advantages of the present invention clearer, preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Experimental methods not indicating specific conditions in the preferred embodiments are generally in accordance with conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, Science Press, 2002) conditions, or as recommended by the manufacturer.

[0017] 1. Preparation of miR-21 complex targeting microglia

[0018] 1. Preparation of fusion peptide K9-S9 of K9 peptide and S9 peptide

[0019] The fusion peptide K9-S9 is formed by directly linking the carboxyl terminal of K9 peptide and the amino terminal of S9 peptide, and the amino acid sequence is KKKKKK-KKKVVMKFLTQQ (SEQ ID No.1).

[0020] The fusion peptide K9-S9 was synthesized on an AB-431A peptide sy...

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Abstract

The invention discloses an miR-21 (microRNA) compound of a target microglial cell, which is formed by combining a fusogenic peptide of a K9 (KKKKKKKKK) peptide and an S9 peptide (VVMKFLTQQ) with miR-21 through the electrostatic interaction. The compound can utilize a guide action of the S9 peptide, and performs specific targeting on specificity of a CX3CR1 accepter on the surface of the microglial cell, and when the S9 peptide acts with the CX3CR1 accepter, the miR-21 is efficiently transfected into the microglial cell at the same time, inhibits activation of the microglial cell, and further inhibits occurrence or deterioration of a central nervous system disease mediated by the microglial cell. The miR-21 compound can be used for preparing a drug for treating the central nervous system disease mediated by the microglial cell, and has a good development and application prospect in the field of nervous system disease treatment.

Description

technical field [0001] The invention belongs to the field of biomedicine, relates to a polypeptide-gene complex, and also relates to a preparation method of the complex and an application in the field of pharmacy. Background technique [0002] As the first line of defense in the central nervous system, microglia play an important role in the inflammatory response. When foreign pathogens invade or cells die, microglia are quickly activated and eliminated, and at the same time release inflammatory factors to mediate the inflammatory response. However, studies have shown that overactivated microglia may accelerate the process of some central nervous system diseases, and the cytotoxic factors released by them will also cause further damage to nerve cells. Therefore, appropriately inhibiting the activity of microglia may be an effective way to treat central nervous system diseases. [0003] MicroRNA (microRNA, miRNA) is a kind of non-coding RNA molecule of about 21-25 nucleotid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K47/42A61P25/28
Inventor 杨曌杨清武
Owner THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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