Method for extracting anthocyanidin by using compound enzyme, and compound enzyme preparation thereof

A compound enzyme preparation, anthocyanin technology, applied in biochemical equipment and methods, enzymes, enzymes and other directions, can solve problems such as low extraction rate, and achieve the effects of improving extraction rate, reducing production cost and reducing energy consumption

Active Publication Date: 2013-04-03
ZHEJIANG HUISONG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Anthocyanins exist in the cells of plants or are adsorbed on the cell walls composed of cellulose, hemifiber and pectin, etc. It is difficult to effectively extract the anthocyanins adsorbed on the cell walls by conventional mechanical or chemical methods, so the extraction rate relatively low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for compound enzyme extracting anthocyanin comprises the following steps:

[0026] (1) Prepare fresh blueberry pulp with a solid-to-liquid ratio of 1:8;

[0027] (2) Stir the fruit pulp prepared in step (1) in a closed container at low speed, control the temperature at 50°C and pH 3.0, add a compound enzyme preparation, and enzymolyze it for 1 hour; the dosage of the compound enzyme preparation is 3mg / g ;

[0028] (3) After the enzymatic hydrolysis is completed, the pomace in the pulp is removed to obtain clarified fruit juice;

[0029] (4) The clarified fruit juice is processed by macroporous resin column chromatography, eluted with acidic ethanol, and spray-dried to obtain anthocyanin products.

[0030] The formula of compound enzyme preparation is: Cellulase 10 wt %, hemicellulase 10 wt %, Protease 10 wt %, pectinase 70 wt %.

[0031] Anthocyanin extraction rate was 93.8%.

Embodiment 2

[0033] The method for compound enzyme extracting anthocyanin comprises the following steps:

[0034] (1) Prepare fresh blueberry pulp with a solid-to-liquid ratio of 1:8;

[0035] (2) Stir the fruit pulp prepared in step (1) at low speed in an airtight container, control the temperature at 60°C and PH at 6.0, add a compound enzyme preparation, and enzymolyze it for 8 hours; the dosage of the compound enzyme preparation is 2mg / g ;

[0036] (3) After the enzymatic hydrolysis is completed, the pomace in the pulp is removed to obtain clarified fruit juice;

[0037] (4) The clarified fruit juice is processed by macroporous resin column chromatography, eluted with acidic ethanol, and spray-dried to obtain anthocyanin products.

[0038] The formula of compound enzyme preparation is: Cellulase 7 wt %, hemicellulase 7 wt %, Protease 7 wt %, pectinase 79 wt %.

[0039] Anthocyanin extraction rate is 96.5%.

Embodiment 3

[0041] The method for compound enzyme extracting anthocyanin comprises the following steps:

[0042] (1) Prepare fresh blueberry pulp with a solid-to-liquid ratio of 1:8;

[0043] (2) Stir the fruit pulp prepared in step (1) in an airtight container at low speed, control the temperature at 45°C, and pH 4.5, add a compound enzyme preparation, and enzymolyze it for 3 hours; the dosage of the compound enzyme preparation is 1mg / g ;

[0044] (3) After the enzymatic hydrolysis is completed, the pomace in the pulp is removed to obtain clarified fruit juice;

[0045] (4) The clarified fruit juice is processed by macroporous resin column chromatography, eluted with acidic ethanol, and spray-dried to obtain anthocyanin products.

[0046] The formula of compound enzyme preparation is: Cellulase 15 wt %, hemicellulase 15 wt %, Protease 15 wt %, pectinase 55 wt %.

[0047] Anthocyanin extraction rate is 95.7%.

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PUM

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Abstract

The invention relates to a method for extracting anthocyanidin by using a compound enzyme, and a compound enzyme preparation thereof. The compound enzyme preparation disclosed by the invention comprises the following components of 7-15 wt% of cellulase, 7-15 wt% of hemicellulase, 7-15 wt% of a protease and 55-79% of pectinase. The method for extracting anthocyanidin by using the compound enzyme comprises the following steps of (1) preparing fresh fruit pulp, and adjusting a solid liquid ratio with water; (2) stirring the fruit pulp obtained from the steps (1) in a sealed container at a low speed, controlling a temperature within 40-60 DEG C and a pH value within 3-6, adding the above compound enzyme preparation, and enzymatic hydrolyzing for 1-8 hours, wherein the usage amount of the compound enzyme is 1 mg / g-3 mg / g; (3) removing fruit residues from the fruit pulp after enzymatic hydrolysis is finished to obtain clear fruit juice; and (4) refining the clear fruit juice by chromatography, and spray-drying to obtain an anthocyanidin product.

Description

technical field [0001] The invention relates to a method for extracting anthocyanins, in particular to a method for extracting anthocyanins with a compound enzyme and a compound enzyme preparation thereof. Background technique [0002] Anthocyanidin (Anthocyanidin), also known as anthocyanin, is a kind of water-soluble natural pigment widely present in plants in nature after it is combined with carbohydrates by glycosidic bonds. It is a biological substance with a special molecular structure. Flavonoids, which mainly exist in the form of anthocyanins in plants, are internationally recognized as the most effective natural antioxidants for eliminating free radicals in the human body. They have very strong biological activity and good absorption, and are 100% biologically effective to the human body , It can be detected in the blood 20 minutes after taking it. [0003] Anthocyanins can nourish the skin and enhance skin immunity. It can not only prevent the early generation of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/00C12N9/42C12N9/50C12N9/26C07D311/62
Inventor 郑猛马全民聂复礼杜大庆
Owner ZHEJIANG HUISONG PHARMA
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