Culture medium for ralstonia solanacearum and preparation method of electrization competent cell of ralstonia solanacearum

A R. solanacearum and culture medium technology, which is applied in the field of preparation of R. solanacearum culture medium and electro-stimulated competent cells of R. The effect of high conversion efficiency

Inactive Publication Date: 2013-04-03
LIAOCHENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effects of chemical agents and integrated agricultural control are not obvious. On the one hand, chemical agent control increases the production cost, and on the other hand, it also pollutes the environment; integrated agricultural control is time-consuming and laborious, and it is difficult to promote

Method used

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Embodiment

[0032] Embodiment: A culture medium of R. solanacearum and a method for preparing competent cells of R. solanacearum electrical stimulation.

[0033] 1. The culture medium of R. solanacearum is prepared from the following raw materials (1 liter medium, pH value 6.8):

[0034] 500g fresh potato; Ca(NO 3 ) 2 .4H 2 O 1.0 g;

[0035] Na 2 HPO 4 .12H 2 O 2.0 grams; Peptone 5.0 grams;

[0036] Yeast extract 1g; sucrose 15g;

[0037] Agar 15g (for solid media; not for liquid media)

[0038] Two, the preparation method of bacterial wilt culture medium:

[0039] (1) Cut 500 grams of fresh potatoes into cubes of 1 cm square, how to put them in a 1-liter beaker filled with 800 ml of distilled water, put them in a microwave oven until boiling, and then cool to room temperature naturally. Filter the potato extract with a 200-mesh nylon mesh, and discard the potato pieces.

[0040] (2) Ca(NO 3 ) 2 .4H 2 O 1.0 g, Na 2 HPO 4 .12H 2 Add 2.0 grams of O, 5.0 grams of peptone, 1 ...

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Abstract

The invention discloses a culture medium for ralstonia solanacearum and a preparation method of an electrization competent cell of the ralstonia solanacearum. The culture medium and the preparation method aim at obtaining the electrization competent cell of the ralstonia solanacearum with higher electrization conversion efficiency. The solution is based on a bacteria cell to be processed by special culture and physicochemical methods to allow the cell to be in a state of easily absorbing foreign DNA (deoxyribonucleic acid); and the prepared competent cell has the higher conversion efficiency through adjustment of a culture medium formula and special processing by a physical method during a cell culture course.

Description

technical field [0001] The invention belongs to the field of prevention and control of agricultural diseases and insect pests, and in particular relates to a culture medium for R. solanacearum and a preparation method for electrically stimulated competent cells of R. solanacearum. Background technique [0002] Bacteria wilt is caused by Ralstia solanaceae ( Ralstonia solanacearum ) is a worldwide bacterial soil-borne disease that is widely distributed in tropical, subtropical and temperate regions. R. solanacearum has a wide range of hosts and can infect more than 200 species of plants in more than 50 families. Once the plants are infected with this pathogen, it will cause a large reduction in crop yield. Due to the global warming caused by the "greenhouse effect", the pathogen has a tendency to spread to high latitude areas, which brings great harm to crop production. Bacterial wilt is a vascular disease that can occur throughout the growing season of crops, and the sympt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 樊颖伦吕山花
Owner LIAOCHENG UNIV
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