Novel high pH induction and carbon dioxide emission reduction coupling microalgae harvesting method
A technology of carbon dioxide and a new method, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of large amount of addition, increased cost, waste of carbon sources, etc., to achieve high-efficiency carbon supplementation, reduce fertilizer costs, The effect of high-efficiency and low-cost harvesting
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Embodiment 1
[0015] The MC-1 strain of Chlorococcum alkaliphilus comes from the South China Sea Institute of Oceanology, Chinese Academy of Sciences. It is used as the algal species, and the medium is modified based on ZSNT. 3 Modified to 5gL -1 , the others are the same, the initial pH value of the medium is 8.2. Use a 3L Erlenmeyer flask to add 2L medium, then inoculate the algae species, the culture OD after inoculation 700 0.2, the light intensity is 100μEm -2 the s -1 , the culture temperature is 25°C, and the photoperiod is 14h:10h (light: dark). Under light conditions, shake well every 4 hours and check the pH of the algae solution. If the pH reaches 9.5-10, inject CO 2 After adjusting the medium to pH 8.2-8.5, stop CO supplementation 2 , so repeatedly, the pH value of the control medium is between 8.2 and 9.5. After the algae liquid has been cultured for 7 days, the concentration of algae cells reaches the level to be harvested at 0.8g / L, and the CO supplementation is stopped...
Embodiment 2
[0018] The MC-1 strain of Chlorococcum alkaliphilus comes from the South China Sea Institute of Oceanology, Chinese Academy of Sciences. It is used as the algae species. The medium is modified based on ZSNT, and NaHCO3 is modified to 5gL -1 , the initial pH of the medium was 8.2. Add 9.6L of culture medium into 20L glass tank reactor, that is, insert 6.4L of Chlorococcum alkaliphilus MC-1 algae liquid (cultivated with the culture medium of this example) into 9.6L of culture medium, and inoculate after culture OD 700 0.3, outdoor natural light cultivation. Under light conditions, stir once every 4 hours, and check the pH of the algae liquid, if the pH reaches 9.5-10, pass CO 2 After adjusting the medium to pH 8.2-9, stop CO supplementation 2 , so repeatedly, the pH value of the control medium is between 8.2 and 9.5. After 3 days of algae culture, the concentration of algae cells reaches the level to be harvested 1g / L, stop supplementing CO 2 , no longer to control the pH o...
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