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Application for drone pupae polypeptide extract

A technology of drone pupae and extract, which is applied in the field of functional research of active components of bee pupae, can solve the problems of tryptophan destruction, destruction, and reduction of nutritional value, and achieve the effect of improving memory and antioxidant function

Active Publication Date: 2013-12-11
ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Acid hydrolysis, alkali hydrolysis and enzyme hydrolysis can be used for protein hydrolysis, but acid hydrolysis leads to the complete destruction of tryptophan, and partial destruction of serine, tyrosine and threonine; alkali hydrolysis causes racemization and reduces nutritional value; enzyme The reaction conditions are mild during hydrolysis, and the nutritional value is guaranteed; therefore, in the prior art, enzymatic hydrolysis is often used to extract drone pupae

Method used

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  • Application for drone pupae polypeptide extract
  • Application for drone pupae polypeptide extract
  • Application for drone pupae polypeptide extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of Polypeptide Extract from Drone Pupa

[0031] Drone pupae (provided by Zhejiang Fengzhiyu Bee Industry Group Co., Ltd.) were washed and homogenized with distilled water, and then dissolved in acetone to degrease. The mass ratio of homogenate to acetone was 1:1.5. The residue was made into a suspension with distilled water, and the mass percent concentration of the suspension was 10% based on drone pupae. Heat the suspension at 90°C for 10 minutes, after cooling, adjust the pH to 8 with 1 mol of NaOH aqueous solution or 1 mol of HCl aqueous solution, add trypsin (400000U / g) and hydrolyze at 45°C for 6 hours, the quality of trypsin is the same as that of drones The mass ratio of the pupae is 0.006:1. After sterilizing at 100°C for 15 minutes, adjust the pH value to 4.5. The supernatant obtained by centrifugation is a polypeptide solution, which is vacuum concentrated and freeze-dried to obtain the drone pupae polypeptide extract.

[0032] The recovery rate ...

Embodiment 2

[0034] Except that the hydrolysis time was 4 hours, other operations were the same as in Example 1 to obtain drone pupae polypeptide extract.

Embodiment 3

[0036] Except that the hydrolysis time was 2 hours, other operations were the same as in Example 1 to obtain drone pupae polypeptide extract.

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Abstract

The invention discloses an application for a drone pupae polypeptide extract, comprising an application for the drone pupae polypeptide extract as a food with a function of memory improvement and / or antioxidation, or for preparing a food with a function of memory improvement and / or antioxidation; an application for the drone pupae polypeptide extract as a healthcare product with a function of memory improvement and / or antioxidation, or for preparing a healthcare product with a function of memory improvement and / or antioxidation; and an application for the drone pupae polypeptide extract for preparing a medicine with a function of memory improvement and / or antioxidation. The drone pupae polypeptide extract has a function of obviously improving memory and a better antioxidation effect, thereby being used as products with a function of memory improvement and / or antioxidation, or for preparing products with a function of memory improvement and / or antioxidation.

Description

technical field [0001] The invention relates to the field of functional research on active components of bee pupae, in particular to the application of drone pupae polypeptide extract. Background technique [0002] my country is one of the countries with the most beekeeping in the world. Bees have a long history of breeding in my country and are rich in resources. my country has a long history of research on the edible value of bee products. At present, it has formed its own unique research system in many aspects, and has developed a variety of products, but there are still many areas to be developed and utilized. [0003] Drone pupae are the unfertilized eggs of bee workers in the drone property, which hatch into larvae and enter the pupal stage. The body of the bee harvested during this period is the drone pupae, which develops by sucking royal jelly, honey and pollen, etc. It has high nutritional value and is a natural food that needs to be developed urgently. According...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/305A23J1/00A23J3/34A61K38/02A61P25/28A61P39/06
Inventor 杨锋童晔玲陈宇戴关海任泽明朱婉萍何娟陈璇
Owner ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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