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Method for producing cordyceps sinensis strain powder through liquid fermentation

A technology of Cordyceps sinensis fungus powder and liquid fermentation, applied in botany equipment and methods, fertilizer mixture, horticulture, etc., can solve the problems of low utilization rate of fermenter, low yield of mycelia, too long fermentation cycle, etc., and improve utilization The effect of improving efficiency and production capacity, shortening fermentation time, and saving production cost

Active Publication Date: 2013-04-03
KINGDOMWAY BIOTECH (JIANGSU) CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is to provide a method for producing Cordyceps sinensis fungus powder by liquid fermentation, which overcomes the problems in the prior art that the liquid fermentation period of Cordyceps sinensis is too long, the yield of mycelium is low, and the utilization rate of the fermentation tank is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Put Cordycepes sinensis (Berk.) Sace strain ACCC50562 from the preserved slant into a sterile slant for cultivation. The cultivation conditions are humidity controlled at 45%, 15°C constant temperature for 15 days, and the slant is covered with fresh spores. . The components of the slant medium are: glucose 15g / L, peptone 0.5g / L, MgSO 4 ·7H 2 O1g / L, KH 2 PO40.5g / L, soybean extract 150mL / L, auxiliary liquid Ⅰ 1mL / L, agar 20g / L, the rest is water, pH5.9, the above components except auxiliary liquid Ⅰ, sterilized at 121°C for 30min, of Solution Ⅰ was sterilized separately at 118°C for 22 minutes and then added to the culture medium. The components of supplementary solution Ⅰ were: thiamine hydrochloride 1g / L, riboflavin 1g / L, vitamin B 30.5g / L, pyridoxine hydrochloride 0.6g / L L, glycine 0.8g / L, biotin 0.08g / L, gibberellin 0.02g / L, NaSeO 30.001g / L, the rest is water. Preparation method of soybean dipping juice: Weigh 150g of potatoes, wash, peel and chop them, add wate...

Embodiment 2

[0034] Put Cordycepes sinensis (Berk.) Sace strain ACCC50562 from the preserved slant into a sterile slant for cultivation. The cultivation conditions are humidity controlled at 50%, 16°C constant temperature for 20 days, and the slant is covered with fresh spores. . The components of the slant medium are: glucose 25g / L, peptone 2g / L, MgSO 4 ·7H 2 O3g / L, KH 2 PO40.75g / L, soybean extract 200mL / L, auxiliary liquid Ⅰ 3g / L, agar 20g / L, the rest is water, pH6.2, the above components except auxiliary liquid Ⅰ, sterilized at 121°C for 30min, of Liquid I was sterilized separately at 118°C for 22 minutes and then added to the culture medium. The components of auxiliary liquid I were: thiamine hydrochloride 3g / L, riboflavin 3g / L, vitamin B30.75g / L, pyridoxine hydrochloride 0.9g / L, Glycine 1g / L, Biotin 0.1g / L, Gibberellin 0.03g / L, NaSeO 3 0.003g / L, the rest is water. Preparation method of soybean dipping juice: Weigh 180g of potatoes, wash, peel and chop them, add water to boil for ...

Embodiment 3

[0040] Put Cordycepes sinensis (Berk.) Sacc strain ACCC50562 from the preserved slant into a sterile slant for cultivation. The cultivation conditions are humidity controlled at 55%, 18°C ​​constant temperature for 25 days, and the slant is covered with fresh spores. . The composition of the slant medium is: glucose 35g / L, peptone 4g / L, MgSO 4 ·7H 2 O5g / L, KH 2 PO 4 1g / L, soybean extract 250mL / L, auxiliary liquid Ⅰ 4mL / L, agar 25g / L, the rest is water, pH 6.6, the above components except auxiliary liquid Ⅰ were sterilized at 121°C for 30min, of which auxiliary liquid Ⅰ was 118°C After being sterilized separately for 22 minutes, the culture medium was added. The components of auxiliary liquid I were: thiamine hydrochloride 4g / L, riboflavin 4g / L, vitamin B31g / L, pyridoxine hydrochloride 1.2g / L, glycine 1.4g / L, Biotin 1.2g / L, Gibberellin 0.04g / L, NaSeO 30.004g / L, the rest is water. Preparation method of soybean dipping juice: Weigh 200g of potatoes, wash, peel and chop them...

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PUM

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Abstract

The invention provides a method for producing cordyceps sinensis strain powder through liquid fermentation, relating to strain powder. The method comprises the following steps of: 1) strain slant culture; 2) rice spore culture; 3) fermentation culture including primary seeding tank culture and secondary fermentation culture; and 4) strain powder preparation. By adopting the method, the seed enlargement frequency can be reduced, the fermentation period is about 120-160 hours, the fermentation time can be effectively shortened, the fermentation tank utilization rate can be improved, the productivity can be increased, the production cost can be saved, the final thalli yield reaches 4-6%, the thalli dry weight reaches 40-60g / L and the requirement of industrial production can be met.

Description

technical field [0001] The invention relates to a fungus powder, in particular to a method for producing the fungus powder of Cordyceps sinensis by liquid fermentation. Background technique [0002] Cordyceps sinensis is a compound of the subsealets and larvae corpses of the ergot fungus Cordyceps sinensis parasitizing on the larvae of bat moths. It is a traditional precious nourishing Chinese medicinal material, which has various functions such as regulating immune system function, anti-tumor, and anti-fatigue. effect. [0003] Cordyceps sinensis grows in cold and high-altitude areas, and its growth environment and conditions are relatively harsh. Due to the continuous destruction of the growth environment of wild Cordyceps, the sharp reduction of insect sources, and the excessive and premature collection by humans, the resources of wild Cordyceps are increasingly scarce. In order to meet market demand, artificial cultivation of Cordyceps sinensis has become the main sour...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/28A01G1/04C05G3/00
Inventor 朱自春陈金卿
Owner KINGDOMWAY BIOTECH (JIANGSU) CO LTD
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