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Method for synthesizing alpha-arbutin by enzymic method through catalysis

An arbutin and enzymatic technology, which is applied in the field of enzymatically catalyzed synthesis of α-arbutin, can solve the problems of development and utilization in the initial stage, and achieve the effects of low cost, low separation cost and high conversion rate

Inactive Publication Date: 2013-03-20
韦慧芳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, α-arbutin has not yet attracted enough attention from the cosmetics and chemical industries in my country, and its development and utilization are just in its infancy.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for enzymatically catalyzing the synthesis of α-arbutin, comprising the steps of:

[0021] One, the preparation of α-arbutin

[0022] (1) Inclined seed culture: Xanthomonas campestris ( Xanthomonas campestris ) CGMCC No.1243 strain, inoculated on the slant medium, cultured at 30°C for 36 hours; the composition mass concentration percentage of the slant medium is as follows: 4% sucrose, 0.5% peptone, 0.5% yeast extract, 0.5% chloride Sodium, 0.1% dipotassium hydrogen phosphate and 2% agar with a pH of 7.0;

[0023] (2) Liquid strain culture: Pick a ring of strains with an inoculation loop, inoculate in a 250mL Erlenmeyer flask containing 50mL of basic fermentation medium, place on a shaker at 30°C, and vibrate at 150r / min for 36 hours to obtain seeds Culture fluid; the composition mass concentration percentage of basic fermentation medium is as follows: 4% sucrose, 0.5% peptone, 0.3% beef extract, 0.1% yeast extract, 0.5% magnesium sulfate and 2% calcium carb...

Embodiment 2

[0038] A method for enzymatically catalyzing the synthesis of α-arbutin, comprising the steps of:

[0039] One, the preparation of α-arbutin

[0040] (1) Inclined seed culture: Xanthomonas campestris ( Xanthomonas campestris) CGMCC No.1243 strain, inoculated on the slant medium, cultured at 35°C for 24 hours; the composition mass concentration percentage of the slant medium is as follows: 6% sucrose, 0.5% peptone, 0.5% yeast extract, 0.5% chloride Sodium, 0.15% dipotassium phosphate and 2% agar with a pH of 7.8;

[0041] (2) Liquid strain culture: pick a ring of strains with an inoculation loop, inoculate in a 250mL Erlenmeyer flask filled with 50mL of basic fermentation medium, place on a shaker at 35°C, and vibrate at 200r / min for 24 hours to obtain seeds Culture fluid; the composition mass concentration percentage of basic fermentation medium is as follows: 6% sucrose, 0.5% peptone, 0.3% beef extract, 0.1% yeast extract, 1.0% magnesium sulfate and 2% calcium carbonate, a...

Embodiment 3

[0056] A method for enzymatically catalyzing the synthesis of α-arbutin, comprising the steps of:

[0057] One, the preparation of α-arbutin

[0058] (1) Inclined seed culture: Xanthomonas campestris ( Xanthomonas campestris ) CGMCC No.1243 strain, inoculated on the slant medium, cultured at 35°C for 30 hours; the composition mass concentration percentage of the slant medium is as follows: 5% sucrose, 0.5% peptone, 0.5% yeast extract, 0.5% chloride Sodium, 0.12% dipotassium phosphate and 2% agar with a pH of 7.5;

[0059] (2) Liquid strain culture: Pick a ring of strains with an inoculation loop, inoculate in a 250mL Erlenmeyer flask filled with 50mL of basic fermentation medium, place on a shaker at 35°C, and vibrate at 180r / min for 30 hours to obtain seeds Culture fluid; the composition mass concentration percentage of basic fermentation medium is as follows: 5% sucrose, 0.5% peptone, 0.3% beef extract, 0.1% yeast extract, 0.6% magnesium sulfate and 2% calcium carbonate, ...

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Abstract

The invention discloses a method for synthesizing alpha-arbutin by an enzymic method through catalysis. The method comprises the following steps of: 1, culturing a slant seed: taking a xanthomonas campestris CGMCC (China General Microbiological Culture Collection Center) NO.1243 strain, and inoculating the xanthomonas campestris CGMCC NO.1243 strain in a slant culture medium for culturing; 2, culturing a liquid strain: selecting a ring strain, inoculating the ring strain in a basic fermentation culture medium, and placing on a table concentrator for oscillating and culturing to obtain a seed culture solution; 3, adding the seed culture solution in the basic fermentation culture medium, oscillating and culturing for 24-48h, freezing, centrifugally collecting thalli, washing the thalli with a PBS (Phosphate Buffer Solution) for 2-3 times, ultrasonically crushing at a low temperature, centrifuging, and taking supernate, namely intracellular crude enzyme; and 4, adding cane sugar and hydroquinone in the intracellular crude enzyme for reacting, and separating and purifying a reaction product to obtain the alpha-arbutin. The method disclosed by the invention has the advantages of high production efficiency, high transformation rate of the hydroquinone, low cost, short process flow and the like.

Description

technical field [0001] The invention belongs to the field of preparation of α-arbutin, in particular to a method for enzymatically catalyzing the synthesis of α-arbutin. Background technique [0002] Arbutin (arbutin), also known as arbutin, arbutin, arbutin, arbutin or myricetin, is a perennial evergreen shrub plant-arbutin leaves from the Rhododendronaceae Ursus The ingredients in the cells have a good skin whitening effect and are widely used in the cosmetics industry. Arbutin can be divided into α-type and β-type according to different structures, β-type, that is, 4-hydroxyphenyl-β-2-D-glucopyranoside, its structural formula is ; and α-arbutin, that is, 4-hydroxyphenyl-α-D-glucopyranoside, its structural formula is . Arbutin has whitening activity, and its whitening mechanism is that arbutin produces competitive and reversible inhibition of tyrosinase, thereby blocking the synthesis of dopa and dopaquinone, thereby inhibiting the production of melanin, and achieving...

Claims

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Application Information

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IPC IPC(8): C12P19/44C12R1/64
Inventor 韦慧芳罗生芳
Owner 韦慧芳
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