Aminoglycosides molecular molecularly imprinted solid-phase extraction column

Abstract

The invention discloses an aminoglycosides molecular molecularly imprinted solid-phase extraction column. Molecularly imprinted polymeric microspheres are filled in a columnar shell, and a preparation method for the molecularly imprinted polymeric microspheres comprises the steps of: taking 3-aminopropyltriethoxysilane or phenyltriethoxysilane as functional monomers, taking tetraethyl orthosilicate as a cross-linking agent and taking aminoglycoside antibiotics as template molecules, performing hydrolytic condensation by adopting a sol-gel method, forming a rigid network structure of an Si-O-Si bond on the surface of activated silica gel to form the polymeric microspheres; and eluting.by using template molecules, so as to obtain the molecularly imprinted polymeric microspheres. The extraction column is used for separating and concentrating target objects before sample pretreatment is performed on residue of veterinary drugs of the aminoglycoside antibiotics, and selectively adsorbing the target objects, and has the advantages of being simple and fast in separation, purification and concentration, little in organic solvent consumption and high in repeatedly used times, and the like.

Description

technical field [0001] The invention relates to the improvement of veterinary drug residue detection technology, in particular to a molecularly imprinted solid-phase extraction column for sample pretreatment before detection of aminoglycoside antibiotic veterinary drug residues in animal foods, and belongs to the technical field of veterinary drug residue detection. [0002] Background technique [0003] Food safety is a major issue related to human health and social harmony. Due to the frequent occurrence of food safety incidents in recent years, countries all over the world have paid great attention to food safety. Developed countries have been paying attention to the issue of veterinary drug residues since the 1970s. At present, countries such as Europe and the United States have established a complete monitoring system for veterinary drug residues, and continue to research and develop efficient and rapid detection methods. entry of animal food. In 2002, my country's M...

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Problems solved by technology

[0009] In view of the complex and tedious sample pretreatment and analysis process in the detection of aminoglycoside antibiotic veterinary drug residues in animal-derived food by liquid chromatography in the prior art, the derivatization conditions are poor in versatility, and the sensitivity needs to be improved. The specific adsorption capacity of substances is not high, and at the same time, the enrichment factor for trace substances is not high, and the requirements for determination cannot be met. The purpose of the present invention is to provide a molecularly imprinted solid phase extraction column. The column is used for the separation and enrichment of the target substance during sample pretreatment before the detection of aminoglycoside antibiotic veterinary drug residues in animal foods. It can selectively adsorb the target substance, and has the advantages of simple and fast separation, purification and enrichment steps. Less consumption of organic solvents, higher number of reuses, etc.

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