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Precise positioning and material taking method of adult rat atrionector

A rat sinoatrial and accurate technology, applied in medical science, veterinary surgery, trocars, etc., can solve the problem of inability to accurately determine adult rat SAN, unclear positioning of rat SAN materials, insufficient positioning, and insufficient materials. Accuracy and other issues to achieve the effect of shortening the collection time, ensuring accuracy, and reducing workload

Inactive Publication Date: 2013-03-13
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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AI Technical Summary

Problems solved by technology

The above studies are not accurate enough from the perspective of positioning and materials
[0005] Since the existing technology cannot accurately determine the position of the adult rat SAN, the location of the rat SAN is not clear in the experiment; in the process of cutting the heart SAN, nearly a thousand slices are often required, and the workload is huge

Method used

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  • Precise positioning and material taking method of adult rat atrionector
  • Precise positioning and material taking method of adult rat atrionector
  • Precise positioning and material taking method of adult rat atrionector

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0051] 1 Materials and methods

[0052] 1.1 Experimental animals

[0053] Twenty secondary Wistar male rats, aged 10 weeks, weighing 250±20g, were provided by the Experimental Animal Center of the Academy of Military Medical Sciences and fed uniformly. Standard laboratory feed was used, free access to food and water, the indoor temperature was 21-23°C, and the relative humidity was 60%.

[0054] 1.2 SAN light microscope collection and fixation of rats

[0055] The right superior vena cava was marked with a suture knot, and the SAN of rats was accurately drawn.

[0056] Firstly, rats were anesthetized by intraperitoneal injection of 1% pentobarbital sodium (50 mg / kg), the thorax was dissected, and the heart was exposed. Tie a knot at the heart end to mark (see figure 1 ) (Reason: The isolated fresh vein wall tissue is very thin, and the surrounding connective tissue is very rich. After the isolation, the vein wall collapses and is mixed with the surrounding connective tissu...

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Abstract

The invention relates to a precise positioning and material taking method of adult rat atrionector. According to the method, the rat atrionector is macroscopically positioned in the proximal part wall of the precava and extends into a junctional zone of the right atrium and the precava, and after the right precava is marked by a body suturing knotting method, the rat heart is taken down along the far end of the precava. The invention also relates to a naked eye precise positioning method of the serial section of the adult rat atrionector. The atrionector subjected to material taking is subjected to the serial section after the fixation, the position and the form of the atrionector on the precava wall is simultaneously observed in the section process through naked eyes, and the atrionector is precisely positioned.

Description

Technical field: [0001] The invention relates to a method for obtaining experimental materials, in particular to a method for quickly locating and accurately obtaining materials for the adult rat sinoatrial node, and also relates to a method for visually and accurately positioning the adult rat sinoatrial node for continuous slices. Background technique: [0002] Rats are one of the most commonly used experimental animals. In medical experimental research, the localization of the sinoatrial node in rats is a technical problem. [0003] The sinoatrial node (sinoatrial node, hereinafter referred to as SAN) is the pacemaker of the normal rhythm of the mammalian heart, and normal SAN function is a prerequisite for the realization of the heart pump function. The anatomical location of the sinoatrial node in rats is concealed and small in size, so it is difficult to find it only by naked eyes. However, it occupies a very important position in the heart's automatic activity. [0...

Claims

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Application Information

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IPC IPC(8): A61D1/00A61B10/02
Inventor 彭瑞云刘燕青高亚兵董霁姚斌伟张静赵黎
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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