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Separation and purification method of gamma-polyglutamic acid

A polyglutamic acid, separation and purification technology, applied in the field of separation and purification of polyglutamic acid, can solve the problems of long process, high organic solvent loss and high production cost, and achieve the effect of removing and improving the quality of the final product

Inactive Publication Date: 2013-03-06
SHANDONG LUBEI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a process for separating and purifying γ-polyglutamic acid from biological fermentation liquid, which is characterized by the combination of membrane precipitation filtration and alcohol analysis, and solves the problem of extracting γ-polyglutamic acid by organic solvent precipitation. The problem of high organic solvent loss caused by glutamic acid and high production cost caused by long process has the advantages of being carried out at room temperature, no phase change, no secondary pollution, simple process, high yield, and high product purity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A kind of separation and purification method of polyglutamic acid of embodiment 1, carry out successively according to the following steps:

[0019] (1) Fermentation broth pretreatment: After the fermentation is finished, stir the γ-polyglutamic acid fermentation broth quickly, add dropwise 50% trichloroacetic acid to adjust the pH to 3.0, add 20g / L, 2% adsorbent diatoms of fermentation acidification solution Thoroughly stir the soil, the ratio of thick and fine diatomite is 1.5:1 (coarse diatomite is 100 mesh, fine diatomite is 200 mesh), add 2.5 times the volume of fermented acidification liquid to dilute with deionized water and pour it into the pre-coated In the suction filtration device of 0.5% diatomaceous earth in the fermented acidification liquid, 0.1MPa pressure circulation suction filtration, until the filtrate turbidity is constant, detection miscellaneous protein content and γ-polyglutamic acid concentration; Then carry out 0.5g / L activated carbon decoloriza...

Embodiment 2

[0022] A kind of separation and purification method of polyglutamic acid of embodiment 2, carry out successively according to the following steps:

[0023] (1) Fermentation broth pretreatment: After the fermentation is finished, stir the γ-polyglutamic acid fermentation broth quickly, add dropwise 50% trichloroacetic acid to adjust the pH to 4.0, add 15g / L, 2% adsorbent diatoms of fermentation acidification solution The soil is fully stirred, the ratio of thick and fine diatomite is 1.0:1 (the coarse diatomite is 100 mesh, and the fine diatomite is 200 mesh), add 3.0 times the volume of fermented acidification liquid to dilute with deionized water, pour it into the pre-coated In the suction filtration device of 0.5% diatomite for fermented acidification liquid, 0.1MPa pressure circulation suction filtration, until the turbidity of the filtrate is constant, detect the miscellaneous protein content and γ-polyglutamic acid concentration; then carry out 1.5g / L activated carbon deco...

Embodiment 3

[0026] A kind of separation and purification method of polyglutamic acid of embodiment 3, carry out successively according to the following steps:

[0027](1) Fermentation broth pretreatment: After the fermentation is finished, stir the γ-polyglutamic acid fermentation broth quickly, add dropwise 50% trichloroacetic acid to adjust the pH to 3.5, add 30g / L, 2% adsorbent diatoms of fermentation acidification solution Thoroughly stir the soil, the ratio of thick and fine diatomite is 2.0:1 (the coarse diatomite is 100 mesh, the fine diatomite is 200 mesh), add 2.0 times the volume of fermented acidification liquid to dilute with deionized water, pour it into the pre-coated In the suction filtration device of 0.5% diatomite for fermented acidification liquid, 0.1MPa pressure circulation suction filtration, until the turbidity of the filtrate is constant, detect the impurity protein content and γ-polyglutamic acid concentration; then carry out 2.0g / L activated carbon decolorization ...

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Abstract

The invention relates to a separation and purification method of polyglutamic acid from fermentation broth. The method comprises the following steps of: regulating the pH value to be between 3.0 and 4.0 by adopting trichloroacetic acid to reduce the viscosity of the fermentation broth; adding activated carbon to decolor and diatomite to adsorb and filter into the fermentation broth; performing super-filtration concentration and alcohol-precipitated purification to the treated fermentation broth sequentially; and drying in vacuum to obtain gamma-polyglutamic acid with purity of more than or equal to 95 percent. The separation and purification method has the advantages of normal temperature performance, no phase change, no secondary pollution, simple process, high yield, high product purity and the like, and is applicable to industrial production.

Description

technical field [0001] The invention relates to a method for separating and purifying polyglutamic acid from fermentation liquid, belonging to the technical field of industrial microorganisms. Background technique [0002] γ-polyglutamic acid (γ-polyglutamic acid, hereinafter referred to as γ-PGA) is produced by the formation of peptide bonds between D-glutamic acid and L-glutamic acid monomers through a-amino and γ-carboxyl groups Polyamide is usually composed of about 5000 glutamic acid monomers, and its molecular weight is generally 100-1000kD. The basic skeleton of γ-PGA is straight-chain fibrous, and there are a large number of highly active free carboxyl groups on the molecular chain to form hydrogen bonds inside or between molecules. It is a water-soluble and biodegradable biopolymer substance. [0003] Polyglutamic acid can be produced by bacteria or chemically synthesized. Compared with the chemically synthesized polyglutamic acid (a-PGA), the polyglutamic acid pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G69/10
Inventor 王克柳王美海
Owner SHANDONG LUBEI PHARMA
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