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Application of rhodamine B thio-bishydrazide derivative as Hg<2+> fluorescent probe

A technology of thiobishydrazide and fluorescent probe, which is applied in the application field of rhodamine B thiobishydrazide derivatives as Hg2+ fluorescent probe, to achieve non-destructive and rapid, extensive social value and economic The effect of efficiency, high selectivity and sensitivity

Inactive Publication Date: 2013-02-27
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] There are atomic absorption spectrometry, inductively coupled plasma mass spectrometry, X-ray fluorescence spectrometry, cold vapor atomic fluorescence spectrometry and other methods to detect metal ions, but they generally require complex multi-stage sample preparation or require sophisticated instruments

Method used

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  • Application of rhodamine B thio-bishydrazide derivative as Hg&lt;2+&gt; fluorescent probe
  • Application of rhodamine B thio-bishydrazide derivative as Hg&lt;2+&gt; fluorescent probe
  • Application of rhodamine B thio-bishydrazide derivative as Hg&lt;2+&gt; fluorescent probe

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Effect test

Embodiment 1

[0025] Using rhodamine B thiobishydrazide derivatives with Hg 2+ Reaction to produce fluorescent rhodamine B-1,3,4-oxadiazole derivatives

[0026] The rhodamine B thiobishydrazide derivative (I) can be prepared according to the method introduced in the applicant's previously applied patent [application number: 201210204864.6].

[0027] Add 50 mL of dichloromethane solution containing 650 mg (1.0 mmol) of thiobishydrazide (I) to 30 mL of 543 mg (2.0 mmol) of HgCl 2 67 mg (0.2 mmol) of tetrabutylammonium bromide was then added to the above mixed solution, and the reaction solution was heated to reflux under stirring, and the reaction was continued for 1.5 hours, and then cooled down. The reaction solution was diluted with 50mL of dichloromethane, allowed to stand for layers, separated, the organic phase was washed with water, dried with anhydrous magnesium sulfate, filtered with suction, and the filtrate was concentrated to obtain 445 mg of a purple-black solid, which was the p...

Embodiment 2

[0035] Rhodamine B Thiobishydrazide Derivatives as Hg 2+ Validation of Fluorescent Probes

[0036] Add 10 mL of DMF / HEPES buffer solution (20 mM HEPES, pH=7.2, 2:3, volume ratio) containing 10 mL of the prepared rhodamine B thiobishydrazide derivative (1) with a micro-syringe. Equivalent Na + ,Mg 2+ ,Al 3+ , K + , Ca 2+ ,Cr 3+ ,Mn 2+ ,Fe 3+ ,Co 2+ , Ni 2+ ,Zn 2+ , Ag + ,Cd 2+ , Ba 2+ ,Pb 2+ ,Hg 2+ and Cu 2+ The ionic water solution was tested by UV-Vis spectrophotometry and fluorescence spectrophotometry respectively.

[0037] The results showed that the effect of rhodamine B thiobishydrazide derivative (I) on Hg 2+ It has very good selectivity, and the contrast before and after adding mercury ions shows that the fluorescence is enhanced by 23 times, and has a strong fluorescence enhancement effect. (See Figure 4 )

Embodiment 3

[0039] Rhodamine B Thiobishydrazide Derivatives as Hg 2+ Hg in serum 2+ Fluorescence Imaging Test

[0040] with HgCl 2 Feed the experimental mice with different doses for 1 or 2 days, then draw blood and centrifuge to get the serum, and use the mercury ion probe rhodamine B thiobishydrazide derivatives within 5 minutes at room temperature to detect the Hg in the serum. 2+ Perform detection and fluorescence imaging.

[0041] see results Figure 8 .

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Abstract

The invention relates to an application of rhodamine B thio-bishydrazide derivative as Hg<2+> fluorescent probe in Hg<2+> detection in water or blood serum, wherein the structural formula of the B thio-bishydrazide derivative is as shown in formula (I); the Hg<2+> detection is realized by utilizing rhodamine B thio-bishydrazide derivative and Hg<2+> to react to generate B-1,3,4-oxadiazole derivative which has a structural formula as shown in formula (I) and is fluorescent. According to the application provided by the invention, the rhodamine B thio-bishydrazide derivative, namely the Hg<2+> fluorescent probe can be selectively reacted with analyte mercury ion, and the sensitivity and strong fluorescence enhancement effect are prominent; non-destructive and fast detection on the mercury ion can be effectively realized, and significant and wide social value and economic benefit are realized.

Description

technical field [0001] The present invention relates to the application of a kind of rhodamine B thiobishydrazide derivatives, especially relates to a kind of rhodamine B thiobishydrazide derivatives as Hg 2+ The application of fluorescent probes uses rhodamine B thiobishydrazide derivatives (structural formula shown in formula (I)) to react with mercury ions to generate fluorescent rhodamine B—1,3,4-oxadiazole derivatives The substance (the structural formula is shown in formula (II)) can be used as Hg 2+ Detection of Hg in water or serum with fluorescent probe 2+ Fluorescence imaging. Background technique [0002] Mercury, a highly toxic transition metal and heavy metal, is one of the most dangerous and widespread pollutants in the environment. Mercury enters the environment in various forms through natural or artificial means, and can enter the human body through air, food, drinking water and cosmetics. Mercury is a non-essential element for the human body and a recog...

Claims

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Application Information

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IPC IPC(8): C09K11/06G01N21/64
Inventor 赵宝祥苗俊英张尚立刘为永李海英
Owner SHANDONG UNIV
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