Endoglucanase coding gene, recombinase and application

Abstract

The invention discloses an endoglucanase coding gene, a recombinase and application, belonging to the technical field of biology. The sequence of the endoglucanase coding gene is shown as SEQ ID NO.1; and the sequence of the recombinase of the endoglucanase is shown as SEQ ID NO.2. Experiment proves that the recombinant endoglucanase provided by the invention has high carboxymethyl cellulose enzyme activity (up to 2245U / mL) and the transparent circle diameter of a colony is 30 times larger than that of the initial strain. The endoglucanase coding gene and the recombinase provided by the invention can be widely used in the degradation of celluloses.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an endoglucanase coding gene, a recombinant enzyme and applications. Background technique [0002] Cellulose is the most abundant carbohydrate resource in the biosphere, accounting for 35%-50% of the dry weight of plants and widely distributed. Cellulose is a long-chain macromolecule, which is connected by glucosides through β-1,4 glycosidic bonds. The molecular weight is generally 50,000-2,500,000, which is equivalent to 300-15,000 glucose units. It is an important storage form of glucose, but Due to the difficulty of degradation, the utilization rate of cellulose resources is very low. Cellulose macromolecules can be degraded by cellulase. Cellulase is an enzyme system. According to its catalytic reaction function, cellulose can be divided into endoglucanase (endo-1,4-β-D-glucanase, EC3.2.1.4, namely C1 enzyme ), exoglucanase (1,4-β-D-glucan cellobilhydrolase or exo-1,4-β-D-glucan...

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Problems solved by technology

Studies on cellulose-degrading bacteria are mostly Bacillus species, and studies on Pantoea ananatis have not been reported

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