Technique for planting mycorrhiza plant root bags in heavy metal contaminated soil
A technology of polluting soil and planting technology, applied in the field of environmental protection, can solve problems such as expensive engineering operation costs, slow growth of plants, and inability to survive, and achieve the effects of expanding the scope of nutrient absorption, promoting growth, and improving effects
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[0015] 1 Pure culture of ectomycorrhizal
[0016] The ectomycorrhizal fungus Laccaria amethystea (bu. Ex Gray) Murr (LA) is preserved by our laboratory. (1) Invert plate Prepare the MMN medium, mix well, and pour plate after autoclaving. The formula of MMN medium is: maltose 3g, glucose 10g, (NH 4 )HPO 4 0.25g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O0.15g,, CaCl 2 0.05g, FeCl 3 ·6H2O0.02g, NaCl0.025g, VB10.1mMg, agar 13g.
[0017] (2) Cutting a single bacterial block Select the MMN plate inoculated with LA, cut out a small piece of 5 mm × 5 mm at the edge of the colony, place it upside down on a new MMN plate, seal it, and incubate at 25°C for 1 month before use.
[0018] 2 Acquisition of mycorrhized plants
[0019] (1) Seedlings of Korean pine (Pinus koraiensis S.) are treated with 30% H 2 o 2 After sterilizing for 30 minutes, rinse with deionized water, then sow in vermiculite, and incubate at 25°C for 8 weeks before use.
[0020] (2) Mycorrhization Place a piece of filt...
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