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Method for rapidly and efficiently rooting pistacia chinensis bunge by taking stem tips and stem segments as explants

An explant and Pistacia chinensis technology, applied in the field of plant biology, can solve the problems of rooting quality adaptability and shortening rooting time, and achieve the effect of shortening rooting time and wide application range.

Inactive Publication Date: 2013-02-06
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its rooting quality, adaptability to materials, and shortening the rooting time need to be improved

Method used

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  • Method for rapidly and efficiently rooting pistacia chinensis bunge by taking stem tips and stem segments as explants

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Embodiment Construction

[0012] The following is a detailed description of the embodiments of the present invention: this embodiment is implemented on the premise of the technical solution of the present invention, and gives detailed implementation modes and specific operation procedures, but the protection scope of the present invention is not limited to the following implementations example.

[0013] A fast and efficient rooting method of Pistacia chinensis taking stem tip and stem segment as explants, including the following steps:

[0014] (1) Material selection: select mature and plump Pistacia seeds;

[0015] (2) Disinfection treatment: first remove the outer hard shell of Pistacia chinensis seeds, then soak the seeds in 75% ethanol solution for 30 seconds, wash 4 times with sterile water, and then put the seeds in 0.1% mercury solution Soak in medium for 9 minutes, shake continuously during this period, wash 4 times with sterile water;

[0016] (3) Inoculation culture: Inoculate the sterilized and rin...

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Abstract

The invention belongs to the technical field of plant biology, particularly relating to a method for rapidly and efficiently rooting pistacia chinensis bunge by taking stem tips and stem segments as explants. In the method, inoculated culture comprises the following specific operation steps of inoculating disinfected and rinsed seeds on an MS culture medium without any hormone, wherein the culture conditions are as follow: 20 to 30 DEG C of temperature, 1,500 to 2,500 lx of illumination intensity and 14 h / d of illumination time; explant culture comprises the following specific operation steps of taking pistacia chinensis bunge seed seedlings; and inoculating the stem tips and the stem segments which are cut off on an ultra-clean workbench onto a rooting culture medium with 1 / 2 WPM inorganic salt, MS organic matter, 1.0 mg / L of IBA (Indole Butyric Acid), 0.2 mg / L of NAA (Naphthyl Acetic Acid), 30 g / L of sucrose and 6.8 g / L of agar powder, wherein the pH of the rooting culture medium is 5.8. According to the method, the rooting time of the pistacia chinensis bunge is further shortened, and a fast, high-efficiency, high-quality, wide-application range method for rooting the pistacia chinensis bunge is obtained.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a rapid and efficient rooting method for Pistacia chinensis with stem tips and stem segments as explants. Background technique [0002] Pistacia ( Pistacia chinensis Bunge) is dioecious, also known as Kaimu, Kaishu, etc., belonging to the deciduous trees of the genus Pistacia in the family Anacardiaceae. It is resistant to drought, salinity, barrenness, strong adaptability, and wide distribution. It is an excellent tree species for greening, timber, ornamental, medicinal and oil. Fresh leaves can extract aromatic oil, which has medicinal effects such as clearing away heat and detoxifying. Roots, stems, leaves, bark and leaves can be used as medicine. The leaves, seeds and bark are rich in tannins, which are good raw materials for producing tannins. Male and female plants have different economic values. Male plants have tough and dense wood, fine texture, and str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 施江辛莉王小燕马香丽郭倩史国安
Owner HENAN UNIV OF SCI & TECH
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