Reconstitutable microecological gel for sea cucumber and abalone culture and preparation method thereof

A micro-ecology and gel technology, applied in applications, animal feed, animal feed, etc., can solve problems such as aquatic diseases, poor stability of microbial preparations and nutritional additives, and inability to effectively use them

Inactive Publication Date: 2013-01-30
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In recent years, the artificial culture of sea cucumber and abalone has developed rapidly, and its main culture forms include factory culture, raft culture, sea bottom sowing culture and cage culture in intertidal zone (see literature: Li Shengkuan et al. Polyculture technology, Aquaculture, 2001, 3:15-17), due to the dilution effect of seawater, the stability of microbial preparations and nutritional additives is poor, the efficiency of being taken into the mouth by sea cucumber and abalone is very low, and most of the dissolved water is lost in seawater or sinks into The bottom mud cannot be effectively used, but it will cause eutrophication of the environment, pollute the water quality, and cause aquatic diseases (see literature: Ni Weifeng et al. Brief introduction to extruded aquatic feed, Scientific Fish Culture, 2012, 4: 71-73)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Gel dry sheet preparation:

[0021] Crush the kelp, add 1.0kg of wet kelp pulp, 0.2L of water, 16g of agar powder, mix well and boil. After lowering the temperature of the above mixture to below 50°C, add 0.13ml of bacterial suspension (100 million spores / ml of Bacillus subtilis suspension) and mix well, pour it into a container and solidify into pieces, the concentration of viable bacteria is about 1×10 4 pcs / g (wet weight); drying at 60°C, viable bacteria concentration ≥ 1×10 4 pieces / g (dry weight).

Embodiment 2

[0023] Gel dry sheet preparation:

[0024] Crush 1.0kg of fresh kelp, add 1kg of fish meal, add 150g of crushed dried asparagus, 3.0L of water, mix well and boil, then cool to below 50°C, add 5.0g of dry powder of Bacillus subtilis at 1×109 / g and mix Evenly, pour it into a container and solidify into a sheet with a thickness of 5mm, and the concentration of viable bacteria is about 1×10 6 pcs / g (wet weight); outdoor sun-dried, viable bacteria concentration ≥ 1×10 6 pieces / g (dry weight).

Embodiment 3

[0026] Gel dry sheet preparation:

[0027] Crush 1.0kg of fresh kelp, 0.25kg of fish meal, 0.05kg of carrageenan, and 1.0L of water, stir and mix well, boil and cool to below 50°C, add 1×10 11 Bacillus subtilis dry powder 230g per gram, mix well and pour into a container to solidify into a gel sheet with a thickness of 5mm, the concentration of live bacteria is about 1×10 10 pcs / g (wet weight); dry in an oven at 60°C, live bacteria concentration ≥ 1×10 10 pieces / g (dry weight). Main nutritional components (weight percentage) of the gel after drying: crude protein content 25.6%, fat 3.0%, fiber 2.2%, carbohydrate 19.9%.

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Abstract

The invention provides reconstitutable microecological gel for sea cucumber and abalone culture and a preparation method thereof and relates to sea cucumber and abalone breeding feed. The gel comprises, by mass ratio, kelp: nutritional agent: gel fixing agent: moisture=1:0-1: 0.016-0.15:0.2-3. The content of beneficial microorganisms is 1*104-1*1010 per gram. During preparation, the kelp is smashed, water and the nutritional agent are added and mixed evenly, the gel fixing agent is added, the mixture is mixed evenly and boiled, beneficial microbial suspension is added and mixed evenly after the temperature drops to below 50 DEG C, and the mixture is pour into a container to be cooled, solidified and dried so as to obtain the reconstitutable microecological gel for the sea cucumber and abalone culture. The reconstitutable microecological gel is formed by fixing various ingredients through algal polysaccharides, and the gel can be ingested by sea cucumbers or abalones after reconstitution and is used for embedding materials good for growth of the sea cucumbers and the abalones such as probiotics and the nutritional agent. The gel can be used effectively because the gel is prevented from being diluted by seawater.

Description

technical field [0001] The invention relates to a sea cucumber and abalone culture feed, in particular to the application of beneficial microorganisms and cell immobilization technology in the sea cucumber and abalone culture field. Background technique [0002] Abalone is a primitive marine shellfish, a single-shell mollusc, with only half of the shell, the shell is thick, flat and wide. Abalone is a traditional and precious ingredient in China, and it is the first of the four seafood delicacies. The wrinkle plate abalone produced in China's Bohai Bay is called wrinkled abalone, and the individual is larger. The southeast coast is called variegated abalone, and the individual is smaller; the semi-patterned abalone and sheep abalone produced in the Xisha Islands are famous edible abalones. It is expensive due to the low natural yield. Now, all abalone-producing countries in the world are developing artificial breeding. China bred variegated abalone seedlings in the 1970s, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/16
Inventor 邵宗泽单大鹏
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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