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Preparation method and application of morelberry effective fractions

A technology of effective parts and physalis, applied in the application of hypoglycemic drugs, the field of preparation of effective part groups, can solve the problems of the application of hypoglycemic drugs for the preparation method of separation of effective part groups that have not been disclosed publicly, and achieve low production cost and high efficacy Exact and easy preparation process

Active Publication Date: 2013-01-23
五源本草(山东)健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above-mentioned documents and patents, etc., have not yet disclosed the preparation method of extracting and separating effective fractions from Physalis pulp and its application in hypoglycemic drugs

Method used

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  • Preparation method and application of morelberry effective fractions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Preparation of Physalis effective fraction group

[0030] Put 20kg of sour berries into an extraction tank, add 15 times the amount of 80% ethanol, extract twice, each time for 1 hour, combine the extracts, recover ethanol, filter, and concentrate to a medicinal solution with a relative density of 1.10 (60°C); Extract three times with dichloromethane, the amount of each time is the same as that of the drug solution, combine the dichloromethane extracts, recover the solvent to dryness, put it on a polyamide column, elute 3BV with 30% ethanol, elute 3BV with 60% ethanol, and combine the elution solution, concentrated and dried to obtain 0.62 kg of effective parts of total terpenoids; the remaining aqueous solution after dichloromethane extraction was evaporated to exhaust the solvent, passed through LSA-21 macroporous adsorption resin column, and eluted with water for 3 times the resin column volume (3BV), Then use 30% ethanol to elute 3BV, 70% ethanol to elute...

Embodiment 2

[0031] Example 2: Preparation of Physalis effective fraction group

[0032] Put 20 kg of persistent calyx physalis in an extraction tank, add 20 times the amount of 30% ethanol, extract 3 times, 2 hours each time, combine the extracts, recover ethanol, filter, and concentrate to a relative density of 1.0 (60°C) Medicinal solution; extract three times with ethyl acetate, the amount of each time is the same as that of the medicinal solution, combine the ethyl acetate extracts, recover the solvent to dryness, put it on a polyamide column, elute 3BV with 10% ethanol, and elute 3BV with 80% ethanol , combined eluents, concentrated and dried to obtain 0.52kg of effective parts of total terpenoids; evaporated the remaining aqueous solution after ethyl acetate extraction to exhaust the solvent, passed through NKA macroporous adsorption resin column, and eluted with water for 3 times of resin column volume (3BV ), then eluted 3BV with 20% ethanol, eluted 2BV with 90% ethanol, combined ...

Embodiment 3

[0033] Example 3: Preparation of Physalis effective fraction group

[0034] Put 10 kg of Physalis root, Physalis stem, and Physalis leaf mixture in an extraction tank, add 10 times the amount of water, extract 3 times, each time for 1.5 hours, combine the extracts, filter, and concentrate to a relative density of 1.20 (60°C ) liquid medicine; extract three times with cyclohexane, the amount of each time is the same as that of the liquid medicine, combine the cyclohexane extracts, recover the solvent to dryness, put it on a polyamide column, elute 3BV with 20% ethanol, and wash with 90% ethanol Remove 3BV, combine the eluents, concentrate and dry to obtain 0.24 kg of effective parts of total terpenoids; evaporate the remaining aqueous solution after cyclohexane extraction to exhaust the solvent, pass through the ADS-17 macroporous adsorption resin column, and elute 3 times the amount of resin with water Column volume (3BV), then elute 3BV with 30% ethanol, 2BV with 70% ethanol,...

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Abstract

The invention relates to a preparation method and an application of morelberry effective fractions. The preparation method comprises the following steps of: extracting morelberry; performing chromatography purification techniques through polyamide, macroporous resins, ion exchange resins and the like to obtain a total steroid effective fraction, a general flavone effective fraction and a total organic acid effective fraction; and combining and uniformly mixing one or two or three of three effective fraction extracts to obtain the morelberry effective fractions. A morelberry effective fraction extract disclosed by the invention can be combined with pharmaceutically-acceptable carriers or other proper excipients, is prepared into an oral administration dosage form or a non-oral administration injection or other dosage forms according to the conventional method, and can be applied to preparation of a medicament for preventing and treating diabetes mellitus.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine preparations, and relates to a preparation method and application of effective fractions of Chinese medicinal materials, in particular to a preparation method of effective fractions of physalis and its application in hypoglycemic drugs. Background technique [0002] With the aging of the world's population, diabetes has become a common and frequently-occurring disease, and its incidence is on the rise in industrialized countries. According to statistics, there are about 150 million diabetic patients in the world, 80% of which are non-insulin-dependent diabetes (type 2), of which there are about 30 million in my country. The number of deaths caused by diabetes complications ranks third after cardiovascular and cerebrovascular diseases and cancer in developed countries, which has attracted great attention from all over the world, and researches on the pathogenesis and drug prevention and ...

Claims

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Application Information

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IPC IPC(8): A61K36/81A61P3/10
Inventor 孔倩倩
Owner 五源本草(山东)健康科技有限公司
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