Nanoparticle with simulated outer cell membrane structure as well as preparation method and application thereof

A technology that imitates the outer membrane of cells and nanoparticles, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of non-specific protein adsorption and cell adhesion, achieve inhibition of protein adsorption and platelet adhesion, and reduce toxicity Side effects, effects of maintaining activity

Inactive Publication Date: 2013-01-23
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are a large number of positive and negative charges in the electrostatic self-assembly system, which easily leads to non-specific protein adsorption and cell adhesion ( Biomaterials , 2002, 23(15): 3193-3201; Biomaterials , 2004, 25(19): 4785-4796; Biomacromolecules , 2009, 10(9): 2436-2445), which limits its application to improve the biocompatibility of materials

Method used

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  • Nanoparticle with simulated outer cell membrane structure as well as preparation method and application thereof
  • Nanoparticle with simulated outer cell membrane structure as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Prepare 0.02 mg / mL chitosan HAc (1%) aqueous solution and 0.02 mg / mLPMA30 aqueous solution. 3 mL of chitosan solution was added dropwise to 5 mL of PMA30 solution stirred at 120 rpm with a syringe at a rate of 0.2 mL / min. Continue stirring for 1 h after the addition is complete. Then, filter with a G3 funnel to remove the large aggregates that may appear, and then CS-PMA30 nanoparticles with a cell-like outer membrane structure with CS as the core and PMA30 as the shell can be obtained. The average particle size is 201.4 nanometers, and the zeta potential is 0.773mV (pH=5.0).

Embodiment 2

[0024] Prepare 0.06 mg / mL chitosan HAc (1%) aqueous solution and 0.06 mg / mLPMA30 aqueous solution. 3 mL of chitosan solution was added dropwise to 5 mL of PMA30 solution stirred at 120 rpm using a syringe at a dropping rate of 0.1 mL / min. Continue stirring for 1 h after the addition is complete. Then, filter with a G3 funnel to remove the large aggregates that may appear, and then CS-PMA30 nanoparticles with a cell-like outer membrane structure with CS as the core and PMA30 as the shell can be obtained. The average particle size is 228.5 nanometers, and the zeta potential value is 2.90mV (pH=5.0).

Embodiment 3

[0026] Prepare 0.2 mg / mL chitosan HAc (1%) aqueous solution and 0.2 mg / mLPMA30 aqueous solution. 3 mL of chitosan solution was added dropwise to 5 mL of PMA30 solution stirred at 120 rpm with a dropping rate of 0.05 mL / min with a syringe. Continue stirring for 1 h after the addition is complete. Then, filter with a G3 funnel to remove the large aggregates that may appear, and then CS-PMA30 nanoparticles with a cell-like outer membrane structure with CS as the core and PMA30 as the shell can be obtained. The average particle size is 263.8 nanometers, and the zeta potential value is 4.47 mV (pH=5.0).

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Abstract

The invention provides a nanoparticle with a simulated outer cell membrane structure, which is formed by electrostatic interaction of polycation and polyanion; the particle diameter of the nanoparticle is 100nm-400nm; the polycation is selected from chitosan, polyethyleneimine, polylysine and dendritic polyurethane; and the polyanion is selected from anionic polymers containing phosphorylcholine groups; the weight ratio of the polycation to the polyanion is (1:5)-(5:1). The nanoparticle has a simple preparation process with a mild condition; the nanoparticle with the simulated outer cell membrane structure is capable of effectively inhibiting protein adsorption and platelet adhesion, and is unlikely to be swallowed by mononuclear macrophages so as to prolong in-vivo circulation time, increase medicine utilization rate and reduce toxic and side effects.

Description

Technical field [0001] The invention relates to a method for preparing nanoparticles with a cell-like outer membrane structure and their applications as gene therapy, controlled drug release, biosensors, etc., and belongs to the technical fields of nanotechnology and biomedical polymer materials. Background technique [0002] Gene therapy is to replace or supplement defective genes in patients with normal function genes to achieve the purpose of treating diseases. It has become an effective way to treat various genetic diseases (including infectious diseases, cancer, etc.). Chitosan has the advantages of degradability, non-toxicity, and non-irritation ( Carbohydrate Polymers , 2010, 79(3): 724-730), has been widely used in biomedicine and other fields. In the field of gene therapy, chitosan acts as a polycation, interacts with negatively charged DNA through electrostatic interactions, and while loading drugs, forms positively charged nanoparticles ( Carbohydrate Polymers , 2005, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K47/32A61K47/34A61K47/36A61K48/00C12N15/63C08J3/14B82Y5/00B82Y40/00
Inventor 宫永宽赵婧宫铭李婷
Owner NORTHWEST UNIV
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