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Prewarning kit for transplant kidney rejection and use method thereof

A technology of rejection reaction and kit, which is applied in the field of early warning kits for renal transplantation rejection, which can solve the problems of great difficulty, complicated and difficult diagnosis of transplant rejection

Inactive Publication Date: 2013-01-02
THE 309TH HOSPITAL OF CHINESE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Transplant rejection is a very complex process, both cellular and humoral immune mechanisms are involved in this process, and there are many factors such as differences in grafts, the impact of surgery, individual differences in transplant recipients, and the application of different immunosuppressive regimens in clinical practice. Participation of allografts complicates and makes the diagnosis of transplant rejection more complicated and difficult
In addition, previous studies have shown that it is difficult to definitively diagnose transplant rejection by detecting a single molecule

Method used

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  • Prewarning kit for transplant kidney rejection and use method thereof
  • Prewarning kit for transplant kidney rejection and use method thereof
  • Prewarning kit for transplant kidney rejection and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] Example 1 Screening of Significant Difference Markers in Early Diagnosis Kit of Transplanted Kidney Rejection

[0106] 1. Collect serum of kidney transplant recipients at multiple time points after transplantation, and analyze and group clinical cases.

[0107] 1.1 Select kidney transplant recipients with preoperative basic diseases or few complications, and no serious complications during the operation, and select kidney transplant recipients at 1 day, 7 days, 14 days, 21 days, 28 days, and 2 months after transplantation. 1. Collect 2ml of whole blood from 3 months to 12 months, let it stand at room temperature for 1 hour, centrifuge at 1000rpm for 10 minutes to collect the supernatant, and store it in a -80°C refrigerator for later use. In addition, the serum of the patients in the acute rejection group after the diagnosis of acute rejection was collected at any time.

[0108]1.2 According to the comprehensive analysis and case screening of serum creatinine, urea nit...

Embodiment 2

[0124] The 35 markers with significant differences obtained in Example 1 were analyzed, and the markers with significant differences in expression levels were analyzed by logistic regression to obtain 4 factors: SCF, sEGFR, sIL2Ralpha and Eotaxin. The early warning and diagnosis system of acute rejection, the statistical analysis shows that the predicted positive rate is 91.4. The area under the ROC diagnostic curve is 97.5%, see appendix figure 1 .

[0125] Wherein, the concrete composition of kit is:

[0126] (1) 96-well filter plate and two sheets of sealing film; purchased from Millipore, Cat. No. MX-PLATE;

[0127] (2) Standards of protein antibodies in the kit; purchased from Millipore, Cat. No. MXH8060, MXH8062, MXH8063, LHSP-8063 and HSCR-8032;

[0128] (3) Quality control control; purchased from Millipore Company, the product numbers are: MXH6060, MXH6062, MXH6063, LHSP-6063 and HSCR-6032;

[0129] (4) Serum matrix; purchased from Millipore Company, the article nu...

Embodiment 3

[0136] Example 3: Composition of the kit for early diagnosis of acute rejection of transplanted kidney

[0137] The 35 markers with significant differences obtained in Example 1 were analyzed, and 14 factors were obtained after logistic regression analysis on the markers with significant differences in expression levels: sTNFR2, Flt3Ligand, Fractalkine, IL1ra, IL2, MDC , MIP1alpha, SDF1alphabeta, TARC, TRAIL, SCF, CCL20, MIP3alpha and XCL1Lymphotactin; statistical analysis shows that the early diagnosis system predicts a positive rate of 98.6%, and the area under the ROC diagnostic curve is 99.8%. figure 2 .

[0138] Wherein, the concrete composition of kit is:

[0139] (1) 96-well filter plate and two sheets of sealing film; purchased from Millipore, Cat. No. MX-PLATE;

[0140] (2) Standards of protein antibodies in the kit; purchased from Millipore, Cat. No. MXH8060, MXH8062, MXH8063, LHSP-8063 and HSCR-8032;

[0141] (3) Quality control control; purchased from Millipore...

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Abstract

The invention relates to a prewarning kit, in particular to a prewarning kit for transplant kidney rejection and a use method thereof. Liquid protein chip luminex detecting technology is used for conducting monitoring and comprehensive analyzing on expressing level of 96 kinds of cell factors / chemotactic factors and receptors of the cell factors / chemotactic factors in 266 portions of serum of patients in 142 cases of acute transplant kidney rejection and transplantation receptors with a stable transplant kidney function. A transplant kidney rejection prewarning system containing at least one united marker is built. Prediction and accuracy of acute rejection is 98.6%, and internal cross validation rate is respectively 97.1% and 97.2%.

Description

[0001] This application is a divisional application of patent application 201110438939.2. technical field [0002] The invention relates to an early-warning kit, in particular to a transplanted kidney rejection early-warning kit and its application method. Background technique [0003] The number of people undergoing dialysis due to chronic renal failure in the world has increased from 426,000 in 1990 to 1.065 million in 2000, and is expected to reach more than 2 million by 2010. This increase in the number of people has led to a rapid increase in global dialysis costs, which has caused a huge economic burden on both developed and developing countries. There are about 1 million uremia patients in my country, 120,000 new patients are added every year, and about 500,000 patients need kidney transplantation every year. At present, my country has carried out a total of nearly 100,000 cases of various organ transplants, becoming the world's second largest organ transplant countr...

Claims

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Application Information

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IPC IPC(8): G01N33/53
Inventor 石炳毅许晓光黄海燕
Owner THE 309TH HOSPITAL OF CHINESE PEOPLES LIBERATION ARMY
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