Traditional Chinese drug microecology composition and preparation method thereof
A technology of traditional Chinese medicine microecology and composition, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., and can solve problems such as prebiotic inhibition
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Embodiment 1
[0020] Chinese medicine components: 10 parts of Astragalus, 5 parts of Atractylodes macrocephala, 5 parts of Fangfeng, 3 parts of Codonopsis pilosula, 5 parts of Coptis chinensis, 3 parts of Daqingye, 5 parts of forsythia, 10 parts of honeysuckle, 5 parts of Houttuynia cordata, 15 parts of Bupleurum, 5 parts of Prunella vulgaris, 5 parts of cinnamon, 5 parts of licorice;
[0021] Preparation:
[0022] (1) The suspensions produced by the slant culture of each strain of Bacillus subtilis, Bacillus licheniformis, Bifidobacterium animalis, lactic acid bacteria and Saccharomyces cerevisiae were expanded and cultured separately, and the liquid complete medium was used for Bacillus subtilis, and the meat was used for Bacillus licheniformis. soup medium, PYG medium for bifidobacteria, MRS medium for lactic acid bacteria, and YPDA medium for Saccharomyces cerevisiae; the culture conditions are 25°C, the culture time is 48h, and the number of viable bacteria is 10 8 a / mL;
[0023] (2)...
Embodiment 2
[0026] Chinese medicine components: Astragalus 15 parts, Atractylodes macrocephala 8 parts, Fangfeng 8 parts, Codonopsis 4 parts, Coptis chinensis 8 parts, Daqingye 4 parts, Forsythia 8 parts, Honeysuckle 15 parts, Houttuynia cordata 8 parts, Bupleurum 18 parts, 8 parts of Prunella vulgaris, 8 parts of cinnamon, 8 parts of licorice;
[0027] Preparation:
[0028](1) The suspensions produced by the slant culture of each strain of Bacillus subtilis, Bacillus licheniformis, Bifidobacterium animalis, lactic acid bacteria and Saccharomyces cerevisiae were expanded and cultured separately, and the liquid complete medium was used for Bacillus subtilis, and the meat was used for Bacillus licheniformis. Soup medium, PYG medium for bifidobacteria, MRS medium for lactic acid bacteria, and YPDA medium for Saccharomyces cerevisiae; the culture conditions are 30°C, the culture time is 30h, and the number of viable bacteria is 6×10 8 a / mL;
[0029] (2) Extraction of active ingredients of t...
Embodiment 3
[0032] Chinese medicine components: 20 parts of Astragalus, 10 parts of Atractylodes macrocephala, 10 parts of Fangfeng, 5 parts of Codonopsis pilosula, 10 parts of Coptis chinensis, 5 parts of Daqingye, 10 parts of forsythia, 20 parts of honeysuckle, 10 parts of Houttuynia cordata, 20 parts of Bupleurum, 10 parts of Prunella vulgaris, 10 parts of cinnamon, 10 parts of licorice;
[0033] Preparation:
[0034] (1) The suspensions produced by the slant culture of each strain of Bacillus subtilis, Bacillus licheniformis, Bifidobacterium animalis, lactic acid bacteria and Saccharomyces cerevisiae were expanded and cultured separately, and the liquid complete medium was used for Bacillus subtilis, and the meat was used for Bacillus licheniformis. Soup medium, PYG medium for bifidobacteria, MRS medium for lactic acid bacteria, and YPDA medium for Saccharomyces cerevisiae; the culture conditions are 32°C, the culture time is 24h, and the number of viable bacteria is 10 9 a / mL;
[0...
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